Quantitative metabolic profiling by 1-dimensional 1 H-NMR analyses: application to plant genetics and functional genomics

Functional Plant Biology : FPB
Annick MoingDominique Rolin

Abstract

Metabolic profiling by 1-dimensional (1-D) 1H-nuclear magnetic resonance (NMR) was tested for absolute quantification of soluble sugars, organic acids, amino acids and some secondary metabolites in fruit, roots and leaves. The metabolite responsible for each peak of the 1H-NMR spectra was identified from spectra of pure compounds. Peak identity was confirmed by the addition of a small amount of commercially-available pure substance. 1H-NMR spectra acquisition was automated. 1H-NMR absolute quantification was performed with a synthesised electronic reference signal and validated by comparison with enzymatic or HPLC analyses; the correlation coefficients between 1H-NMR data and enzymatic or HPLC data were highly significant. Depending on the species and tissues, 14-17 metabolites could be quantified with 15-25 min acquisition time. The detection limit was approximately 1-9 µg in the NMR tube, depending on the compound. Quantitative data were used for (1) a genetic study of strawberry fruit quality, (2) a functional study of tomato transformants overexpressing hexokinase and (3) a study of Arabidopsis phosphoenolpyruvate carboxylase transformants with several lines showing decreased activity of the enzyme. Biochemical phenotyping ...Continue Reading

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Citations

Mar 5, 2019·Metabolomics : Official Journal of the Metabolomic Society·Simon RoquesAnnick Moing
Jun 3, 2005·Phytochemical Analysis : PCA
Feb 28, 2007·Plant, Cell & Environment·Imene GharbiRenaud Brouquisse
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Aug 23, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ana Cristina Abreu, Ignacio Fernández
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Jul 14, 2018·The Plant Journal : for Cell and Molecular Biology·Pawel JurkiewiczHenri Batoko
Mar 5, 2019·Metabolomics : Official Journal of the Metabolomic Society·Stéphane BernillonAnnick Moing
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