Quantitative metabolome, proteome and transcriptome analysis of midgut and fat body tissues in the mountain pine beetle, Dendroctonus ponderosae Hopkins, and insights into pheromone biosynthesis

Insect Biochemistry and Molecular Biology
Christopher I KeelingJörg Bohlmann

Abstract

Bark beetles (Coleoptera: Scolytinae) are pests of many forests around the world. The mountain pine beetle (MPB), Dendroctonus ponderosae Hopkins, is a significant pest of western North American pine forests. The MPB is able to overcome the defences of pine trees through pheromone-assisted aggregation that results in a mass attack of host trees. These pheromones, both male and female produced, are believed to be biosynthesized in the midgut and/or fat bodies of these insects. We used metabolite analysis, quantitative proteomics (iTRAQ) and transcriptomics (RNA-seq) to identify proteins and transcripts differentially expressed between sexes and between tissues when treated with juvenile hormone III. Juvenile hormone III induced frontalin biosynthesis in males and trans-verbenol biosynthesis in females, as well as affected the expression of many proteins and transcripts in sex- and tissue-specific ways. Based on these analyses, we identified candidate genes involved in the biosynthesis of frontalin, exo-brevicomin, and trans-verbenol pheromones.

References

Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·S J SeyboldG J Blomquist
Dec 26, 2001·Nucleic Acids Research·Ron EdgarAlex E Lash
Dec 4, 2002·Annual Review of Entomology·Steven J Seybold, Claus Tittiger
Jul 25, 2003·Insect Biochemistry and Molecular Biology·Lana S BarkawiSteven J Seybold
Nov 25, 2003·Analytical Chemistry·Alexey I NesvizhskiiRuedi Aebersold
Sep 24, 2004·Molecular & Cellular Proteomics : MCP·Philip L RossDarryl J Pappin
Oct 6, 2004·Genome Biology·Robert C GentlemanJianhua Zhang
Oct 26, 2005·BMC Genomics·Ian P ShadforthConrad Bessant
Oct 19, 2006·Insect Biochemistry and Molecular Biology·Pamela SandstromClaus Tittiger
May 1, 2008·Nucleic Acids Research·Stefan GötzAna Conesa
Sep 5, 2008·Journal of Economic Entomology·John H BordenJean Pierre Lafontaine
Mar 12, 2009·Die Naturwissenschaften·Anna B GilgGary J Blomquist
Jun 19, 2010·PloS One·Robert ParkerLeonard J Foster
Aug 24, 2010·Insect Biochemistry and Molecular Biology·Gary J BlomquistClaus Tittiger
Nov 23, 2010·Proceedings of the National Academy of Sciences of the United States of America·Sungbeom LeeDorothea Tholl
Apr 5, 2011·Molecular Ecology·Catherine I CullinghamDavid W Coltman
May 21, 2011·BMC Genomics·Bo ZhuGu-Lei Jin
Dec 1, 2011·Nucleic Acids Research·Marco PuntaRobert D Finn
Jun 12, 2012·Nucleic Acids Research·Marc LohseBjörn Usadel
Aug 29, 2012·Proceedings of the National Academy of Sciences of the United States of America·Yue QiuRené Feyereisen
Oct 13, 2012·Bioinformatics·Limin FuWeizhong Li
Oct 17, 2012·Bioinformatics·Evguenia KopylovaHélène Touzet
Nov 6, 2012·Nucleic Acids Research·Sarah W BurgeAlex Bateman
Nov 30, 2012·Nucleic Acids Research·Tanya BarrettAlexandra Soboleva
Nov 30, 2012·Nucleic Acids Research·Christian QuastFrank Oliver Glöckner
Mar 19, 2013·Journal of Proteomics·Salvador Martínez-BartoloméJuan P Albar
Mar 30, 2013·Genome Biology·Christopher I KeelingJoerg Bohlmann
Oct 30, 2013·Proceedings of the National Academy of Sciences of the United States of America·Christopher I KeelingJoerg Bohlmann

❮ Previous
Next ❯

Citations

Mar 23, 2018·Natural Product Reports·Tobias Engl, Martin Kaltenpoth
Jul 26, 2018·Journal of Chemical Ecology·Christine C ChiuJoerg Bohlmann
Apr 27, 2020·Archives of Insect Biochemistry and Physiology·Umut ToprakGözde Güney
Mar 21, 2018·Proceedings of the National Academy of Sciences of the United States of America·Christine C ChiuJoerg Bohlmann
Dec 29, 2020·Current Opinion in Insect Science·Gary J BlomquistChristopher I Keeling
Dec 7, 2017·Current Opinion in Insect Science·Claus Tittiger, Gary J Blomquist
Oct 7, 2021·Natural Product Reports·Mariana AvalosDana Ulanova

❮ Previous
Next ❯

Related Concepts

Related Feeds

Barrett Esophagus

Barrett’s esophagus if a serious complication of gastroesophageal reflux disease during which the normal esophageal lining changes to tissue that resembles intestinal lining. Here is the latest research.

Cardiac Glycosides

Cardiac glycosides are a diverse family of naturally derived compounds that bind to and inhibit na+/k+-atpase. Discover the latest research on cardiac glycosides heres.