PMID: 3760234Sep 1, 1986Paper

Quantitative method for determining serum alkaline phosphatase isoenzyme activity II. Development and clinical application of method for measuring four serum alkaline phosphatase isoenzymes

Journal of Clinical Pathology
M D ShephardR N Walmsley

Abstract

A method for quantitating the liver, bone, intestinal and placental alkaline phosphatase activity of serum, using an algorithm for converting selective inactivation by guanidine hydrochloride, L-phenylalanine, and heat into equivalent isoenzyme activity is described. The method can individually quantify mixtures of isoenzymes to within a margin of 3%; it has acceptable reproducibility and has been used to develop both age and sex related reference ranges. Analysis time is about 30 minutes. The clinical reliability of this method has been shown in a study of 101 patients, in 79% of whom isoenzyme results were compatible with the final clinical diagnosis; in 10% a clinical diagnosis resulted from isoenzyme analysis, and in a further 11% the source of the increased alkaline phosphatase activity was identified and supported by electrophoresis, with a definite clinical diagnosis yet to be made.

References

May 17, 1976·Clinica Chimica Acta; International Journal of Clinical Chemistry·J StĕpánJ Kolár
Oct 1, 1977·Clinica Chimica Acta; International Journal of Clinical Chemistry·K B WhitakerD W Moss
Oct 15, 1985·Clinica Chimica Acta; International Journal of Clinical Chemistry·J J StĕpánV Pacovský
Nov 1, 1983·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·H E Solberg
Mar 1, 1982·Critical Reviews in Clinical Laboratory Sciences·P M Crofton
May 1, 1965·Journal of Clinical Pathology·F C NEALES POSEN

❮ Previous
Next ❯

Citations

Mar 15, 1988·Clinica Chimica Acta; International Journal of Clinical Chemistry·R N SeabrookJ P Luzio
Jul 15, 1991·Clinica Chimica Acta; International Journal of Clinical Chemistry·I Ramasamy
Jun 30, 1992·Clinica Chimica Acta; International Journal of Clinical Chemistry·B R ChamberlainP R Pannall
Oct 6, 2011·Journal of Pediatric Gastroenterology and Nutrition·Robert C De LisleMegan Boyd
Jul 3, 2013·Ecotoxicology and Environmental Safety·María Mercedes IummatoAngela Beatriz Juárez
Nov 20, 2002·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·Giuseppe PerinettiGiuseppe Spoto
Jul 20, 2007·Biological Trace Element Research·Aleksandra CerovicAhmed El-Sohemy
Jan 1, 2001·Indian Journal of Clinical Biochemistry : IJCB·M S GhadgeP Jagannath
Aug 27, 2005·Clinical Chemistry·Masakazu IzumiMasato Maekawa
Jul 3, 2008·Journal of the American Animal Hospital Association·Janean L FidelShannon P Holmes
Jul 1, 1993·Annals of Clinical Biochemistry·C P Price

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.