PMID: 5900215Jan 1, 1966

Quantitative micro determination and isolation of plasmalogen aldehydes as 2,4-dinitrophenylhydrazones.

Journal of Lipid Research
I Katz, M Keeney

Abstract

A micro spectrophotometric procedure for the quantitative determination of plasmalogen aldehydes is described which utilizes simultaneous methanolysis and formation of 2,4-dinitrophenylhydrazones. After isolation of the hydrazones by thin-layer chromatography, the aldehydes can be regenerated, reduced, acetylated, and then analyzed by gas-liquid chromatography. Identification of the plasmalogen aldehydes obtained from rumen holotrich protozoa is described.

Related Concepts

Aldehydes
Metazoa
Thin Layer Chromatography
Dinitrophenols
Microchemistry
Phenylhydrazines
Phosphatidylethanolamines
Plasmalogens
Spectrophotometry
Eukaryota

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