Quantitative microscopy of the Drosophila ovary shows multiple niche signals specify progenitor cell fate

Nature Communications
Wei DaiDenise Montell

Abstract

Adult stem cells commonly give rise to transit-amplifying progenitors, whose progeny differentiate into distinct cell types. It is unclear if stem cell niche signals coordinate fate decisions within the progenitor pool. Here we use quantitative analysis of Wnt, Hh, and Notch signalling reporters and the cell fate markers Eyes Absent (Eya) and Castor (Cas) to study the effects of hyper-activation and loss of niche signals on progenitor development in the Drosophila ovary. Follicle stem cell (FSC) progeny adopt distinct polar, stalk, and main body cell fates. We show that Wnt signalling transiently inhibits expression of the main body cell fate determinant Eya, and Wnt hyperactivity strongly biases cells towards polar and stalk fates. Hh signalling independently controls the proliferation to differentiation transition. Notch is permissive but not instructive for differentiation of multiple cell types. These findings reveal that multiple niche signals coordinate cell fates and differentiation of progenitor cells.

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Citations

Nov 6, 2018·Development·Rebecca P Kim-Yip, Todd G Nystul
Jan 26, 2020·Cells·Matthew Antel, Mayu Inaba
Jan 29, 2020·G3 : Genes - Genomes - Genetics·Carrie A WhittleCassandra G Extavour
Mar 4, 2020·PLoS Computational Biology·Sebastian M BernasekLuís A N Amaral
Jul 28, 2019·Nature Communications·Dong-Yuan ChenDavid Bilder
Aug 8, 2019·Molecular Biology of the Cell·Abhinava K MishraDenise J Montell
Oct 14, 2018·The Journal of Cell Biology·Katharine A WhiteDiane L Barber
Feb 13, 2020·Signal Transduction and Targeted Therapy·Liqun YangHongjuan Cui
Nov 8, 2020·Nature Communications·Katja RustTodd G Nystul
Apr 17, 2020·Signal Transduction and Targeted Therapy·Liqun YangHongjuan Cui
Feb 4, 2022·Journal of Cell Science·Indrayani Waghmare, Andrea Page-McCaw

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