Quantitative molecular diagnosis of levamisole resistance in populations of Haemonchus contortus

Experimental Parasitology
Jessica Maria Leite Dos SantosJomar Patrício Monteiro

Abstract

Parasitism by Haemonchus contortus is one of the main limiting factors in small ruminant production around the globe. Although several studies suggest the use of integrated management practices, these parasites have been controlled essentially with synthetic anthelmintic drugs. The resistance mechanism against the imidazothiazole derivative levamisole in Haemonchus contortus has not been fully described. Recently, resistance was associated with a 63bp deletion in the Hco-acr-8b gene that encodes a subunit for a nicotinic acetylcholine receptor. This study aimed to standardize a real time PCR (qPCR) protocol for levamisole resistance diagnosis in H. contortus populations based on this polymorphism and use it to characterize 23 field H. contortus populations obtained from different localities of Ceará State, Northeast Brazil. In addition, two populations of H. contortus were used as a standard of susceptibility and resistance, Inbred Strain Edinburgh (ISE) and Kokstad, respectively. Larval development tests (LDT) were performed on five field isolates and both EC50 and EC95 were estimated. LDT EC95 values provided a wider interval between susceptible and resistant populations than EC50 values (EC95 = 1.96-57.93 μM; EC50 = 0.05-0.3...Continue Reading

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Citations

Nov 21, 2020·Tropical Animal Health and Production·Muhammad NaeemNabila Roohi
Feb 5, 2021·Animals : an Open Access Journal From MDPI·Konstantinos V ArsenopoulosElias Papadopoulos
Apr 3, 2021·International Journal for Parasitology. Drugs and Drug Resistance·Paulius BaltrušisJohan Höglund

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