Quantitative multi-image analysis for biomedical Raman spectroscopic imaging

Journal of Biophotonics
Martin HedegaardMolly M Stevens

Abstract

Imaging by Raman spectroscopy enables unparalleled label-free insights into cell and tissue composition at the molecular level. With established approaches limited to single image analysis, there are currently no general guidelines or consensus on how to quantify biochemical components across multiple Raman images. Here, we describe a broadly applicable methodology for the combination of multiple Raman images into a single image for analysis. This is achieved by removing image specific background interference, unfolding the series of Raman images into a single dataset, and normalisation of each Raman spectrum to render comparable Raman images. Multivariate image analysis is finally applied to derive the contributing 'pure' biochemical spectra for relative quantification. We present our methodology using four independently measured Raman images of control cells and four images of cells treated with strontium ions from substituted bioactive glass. We show that the relative biochemical distribution per area of the cells can be quantified. In addition, using k-means clustering, we are able to discriminate between the two cell types over multiple Raman images. This study shows a streamlined quantitative multi-image analysis tool for...Continue Reading

References

Dec 9, 2003·Applied Spectroscopy·Chad A Lieber, Anita Mahadevan-Jansen
Feb 22, 2012·Biophysical Journal·Katharina KleinJürgen Schlegel
Jul 4, 2012·Laboratory Investigation; a Journal of Technical Methods and Pathology·Benjamin BirdMax Diem
Jul 24, 2013·Applied Spectroscopy·Iwan W Schie, Thomas Huser
Sep 5, 2013·Proceedings of the National Academy of Sciences of the United States of America·Kenny KongIoan Notingher
Feb 12, 2015·The Analyst·Thomas C von ErlachMolly M Stevens
Apr 2, 2015·Proceedings of the National Academy of Sciences of the United States of America·Hélène AutefageMolly M Stevens

❮ Previous
Next ❯

Citations

Mar 23, 2017·Nature Communications·Charalambos KallepitisMolly M Stevens
May 15, 2018·Integrative Biology : Quantitative Biosciences From Nano to Macro·Stefano FornasaroValter Sergo
Apr 12, 2017·Current Protocols in Neuroscience·Christian A Combs, Hari Shroff
Nov 19, 2019·Frontiers in Bioengineering and Biotechnology·Mads S BergholtMichael B Albro
Apr 10, 2018·Frontiers in Chemistry·Jana S SegmehlIngo Burgert
Apr 8, 2021·Annual Review of Analytical Chemistry·Cassio LimaRoyston Goodacre

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.