Quantitative multiplex CARS spectroscopy in congested spectral regions

The Journal of Physical Chemistry. B
Hilde A RiniaMichiel Müller

Abstract

A novel procedure is developed to describe and reproduce experimental coherent anti-Stokes Raman scattering (CARS) data, with particular emphasis on highly congested spectral regions. The approach, exemplified here with high-quality multiplex CARS data, makes use of spontaneous Raman scattering results. It is shown that the underlying vibrational Raman response can be retrieved from the multiplex CARS spectra, so that the Raman spectrum can be reconstituted, provided an adequate signal-to-noise ratio (SNR) is present in the experimental data and sufficient a priori knowledge of the vibrational resonances involved exists. The conversion of CARS to Raman data permits a quantitative interpretation of CARS spectra. This novel approach is demonstrated for highly congested multiplex CARS spectra of adenosine mono-, di-, and triphosphate (AMP, ADP, and ATP), nicotinamide adenine dinucleotide (NAD+), and small unilamellar vesicles (SUVs) of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC). Quantitative determination of nucleotide concentrations and composition analysis in mixtures is demonstrated.

References

Jan 1, 1999·Chemistry and Physics of Lipids·R Mendelsohn, D J Moore
Jun 29, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Alan P KennedyJi-Xin Cheng
Jul 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Henk-Jan van ManenCees Otto
Dec 1, 2007·Optics Letters·J X ChengX S Xie

❮ Previous
Next ❯

Citations

Jul 22, 2011·Journal of the American Chemical Society·Wei MaHong-Yuan Chen
Apr 21, 2011·The Journal of Physical Chemistry. B·Christian W FreudigerX Sunney Xie
Dec 4, 2010·Science·Brian G SaarX Sunney Xie
May 29, 2010·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Nadine VoglerJürgen Popp
Sep 19, 2009·Vibrational Spectroscopy·Han-Wei WangJi-Xin Cheng
Oct 31, 2015·Annual Review of Biomedical Engineering·Chi ZhangJi-Xin Cheng
Sep 5, 2007·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Michiel Müller, Andreas Zumbusch
Dec 21, 2006·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Hilde A RiniaErik M Vartiainen
Mar 1, 2012·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Xu ZhangX Sunney Xie
Jun 17, 2016·Annual Review of Analytical Chemistry·Chien-Sheng Liao, Ji-Xin Cheng
Apr 30, 2011·The Journal of Physical Chemistry. B·James P R DayMischa Bonn
Nov 10, 2006·Journal of Biomedical Optics·Hilde A RiniaMichiel Müller
Oct 25, 2006·Applied Spectroscopy·Zachary D SchultzLee J Richter
Nov 3, 2020·Journal of Raman Spectroscopy : JRS·Alejandro De la CadenaDario Polli
Sep 2, 2010·The Journal of Physical Chemistry. B·Paul M DonaldsonDavid R Klug
Sep 5, 2019·Analytical Chemistry·Karim AljakouchSamir F El-Mashtoly
Jul 17, 2020·The Journal of Physical Chemistry. B·Teemu HärkönenErik M Vartiainen
Apr 14, 2007·Science·Dmitry PestovMarlan O Scully

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.