Quantitative nephelometric determination of Haemophilus influenzae antigen in body fluids.

Journal of Clinical Microbiology
T CaceciA Daniel

Abstract

Nephelometry, an immunological technique widely used for the quantification of blood proteins, was adapted to provide a quantitative method of detecting Haemophilus influenzae capsular antigen in body fluids. Using specific antiserum directed against H. influenzae capsular antigen, samples of serum, cerebrospinal fluid, urine, and joint fluid from 38 cases of H. influenzae infections were analyzed. The results were compared for reliability to counterimmunoelectrophoresis, a widely used diagnostic tool. The nephelometric technique has the same advantages of speed and specificity as counterimmunoelectrophoresis and provides the clinician and researcher with a quantitative method that is as reliable as the qualitative counterimmunoelectrophoresis procedure. The method allowed directly quantitative readouts on patient specimens, with no necessity for serial dilutions or densitometric readings.

References

Aug 1, 1977·The Journal of Infectious Diseases·D M Granoff, G A Nankervis
Jan 1, 1978·Scandinavian Journal of Infectious Diseases·P Gaustad, R Bolle
Feb 1, 1973·American Journal of Clinical Pathology·R F RitchieC Larson

❮ Previous
Next ❯

Citations

Jul 28, 1983·The American Journal of Medicine·J K Todd

❮ Previous
Next ❯

Related Concepts

Related Feeds

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.