Abstract
An on-line in vitro perfusion technique is described that allows the continuous quantification of cellular glucose metabolism in vitro. Using biosensor technology, we measure glucose and lactate metabolism at a minute-to-minute time resolution for periods up to several days. The application of our perfusion-detection technique for in vitro monitoring is demonstrated in a wide variety of cells, including primary neuronal and astroglia cultures, yeast cells, and human lymphocytes. The method shows that variations in oxygen delivery or exposure to a noncompetitive pseudosubstrate (here 2-deoxyglucose) affects normal glucose metabolism. The innovative advantage of the present system is that, in contrast to other devices including a recently described system, metabolism per cell can be quantified. The potential of in vitro on-line monitoring is discussed for application in studying normal and abnormal metabolism, toxic and nontoxic drug effects, and human tissue biopsies.
References
Oct 1, 1993·Brain Research·R DringenB Hamprecht
Jan 1, 1993·Antonie van Leeuwenhoek·J P van DijkenJ T Pronk
Dec 1, 1996·Neuroscience Research·A TaberneroJ M Medina
Jan 1, 1997·Biosensors & Bioelectronics·W A KapteinJ Korf
Aug 5, 1998·Acta neurochirurgica·J ValtyssonL Hillered
Oct 21, 1998·Developmental Neuroscience·A Schurr, B M Rigor
Nov 4, 1998·Analytical Biochemistry·P G ArthurC M Wakeford
Nov 6, 1998·Journal of Biotechnology·T Noll, M Biselli
Dec 9, 1998·Analytical Chemistry·W A KapteinJ Korf
Aug 31, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·P J Magistretti, L Pellerin
Mar 7, 2001·The Journal of Physiology·H L CaterL E Sundstrom
May 4, 2001·Journal of Immunological Methods·H GloecknerH D Lemke
Aug 9, 2001·Biotechnology and Bioengineering·H P BonariusE Heinzle
Oct 6, 2001·Biotechnology Progress·H Gloeckner, H D Lemke
Oct 13, 2001·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·D Attwell, S B Laughlin
Dec 18, 2001·Journal of Neuroscience Research·G Leegsma-VogtJ Korf
May 16, 2002·Analytical Biochemistry·Carmen Wiley, Craig Beeson
Aug 8, 2002·Fundamental & Clinical Pharmacology·Ian D WakefieldJean-Pierre Valentin
Sep 25, 2002·Brain Research·Luca CuculloDamir Janigro
Apr 5, 2003·Journal of Neurochemistry·Jan Bert GramsbergenJakob Korf
Aug 7, 2003·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Gea Leegsma-VogtJakob Korf
Jan 31, 2004·Analytical Chemistry·Sven E EklundDavid E Cliffel
Citations
Jan 1, 2011·Journal of Mathematical Chemistry·Momchil VelkovskyJohn P Wikswo
Jul 30, 2008·Analytical Chemistry·John Paul UrbanskiTodd Thorsen
Sep 5, 2006·IEE Proceedings. Nanobiotechnology·J P WikswoM Velkovsky
Oct 3, 2007·Analytical and Bioanalytical Chemistry·G A Posthuma-TrumpieJ Korf
Jun 5, 2007·Biochimica Et Biophysica Acta·Geertruida A Posthuma-TrumpieWillem J H van Berkel
Jul 16, 2013·IEEE Transactions on Biomedical Circuits and Systems·Cristina BoeroSandro Carrara
Mar 28, 2007·Analytica Chimica Acta·Joseph WangGreg E Collins
Jun 7, 2014·Biosensors & Bioelectronics·Cristina BoeroGiovanni De Micheli