Quantitative on-line monitoring of cellular glucose and lactate metabolism in vitro with slow perfusion

Analytical Chemistry
Gea Leegsma-VogtJakob Korf

Abstract

An on-line in vitro perfusion technique is described that allows the continuous quantification of cellular glucose metabolism in vitro. Using biosensor technology, we measure glucose and lactate metabolism at a minute-to-minute time resolution for periods up to several days. The application of our perfusion-detection technique for in vitro monitoring is demonstrated in a wide variety of cells, including primary neuronal and astroglia cultures, yeast cells, and human lymphocytes. The method shows that variations in oxygen delivery or exposure to a noncompetitive pseudosubstrate (here 2-deoxyglucose) affects normal glucose metabolism. The innovative advantage of the present system is that, in contrast to other devices including a recently described system, metabolism per cell can be quantified. The potential of in vitro on-line monitoring is discussed for application in studying normal and abnormal metabolism, toxic and nontoxic drug effects, and human tissue biopsies.

References

Jan 1, 1993·Antonie van Leeuwenhoek·J P van DijkenJ T Pronk
Oct 21, 1998·Developmental Neuroscience·A Schurr, B M Rigor
Nov 4, 1998·Analytical Biochemistry·P G ArthurC M Wakeford
Aug 31, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·P J Magistretti, L Pellerin
Oct 13, 2001·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·D Attwell, S B Laughlin
May 16, 2002·Analytical Biochemistry·Carmen Wiley, Craig Beeson
Aug 8, 2002·Fundamental & Clinical Pharmacology·Ian D WakefieldJean-Pierre Valentin
Aug 7, 2003·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Gea Leegsma-VogtJakob Korf

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Citations

Jan 1, 2011·Journal of Mathematical Chemistry·Momchil VelkovskyJohn P Wikswo
Jul 30, 2008·Analytical Chemistry·John Paul UrbanskiTodd Thorsen
Oct 3, 2007·Analytical and Bioanalytical Chemistry·G A Posthuma-TrumpieJ Korf
Jun 5, 2007·Biochimica Et Biophysica Acta·Geertruida A Posthuma-TrumpieWillem J H van Berkel
Jul 16, 2013·IEEE Transactions on Biomedical Circuits and Systems·Cristina BoeroSandro Carrara
Mar 28, 2007·Analytica Chimica Acta·Joseph WangGreg E Collins
Jun 7, 2014·Biosensors & Bioelectronics·Cristina BoeroGiovanni De Micheli

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