Quantitative Online Liquid Chromatography-Surface-Enhanced Raman Scattering (LC-SERS) of Methotrexate and its Major Metabolites

Analytical Chemistry
Abdu SubaihiRoyston Goodacre

Abstract

The application of Raman spectroscopy as a detection method coupled with liquid chromatography (LC) has recently attracted considerable interest, although this has currently been limited to isocratic elution. The combination of LC with rapidly advancing Raman techniques, such as surface-enhanced Raman scattering (SERS), allows for rapid separation, identification and quantification, leading to quantitative discrimination of closely eluting analytes. This study has demonstrated the utility of SERS in conjunction with reversed-phase liquid chromatography (RP-LC), for the detection and quantification of the therapeutically relevant drug molecule methotrexate (MTX) and its metabolites 7-hydroxy methotrexate (7-OH MTX) and 2,4-diamino-N(10)-methylpteroic acid (DAMPA) in pure solutions and mixtures, including spikes into human urine from a healthy individual and patients under medication. While the RP-LC analysis developed employed gradient elution, where the chemical constituents of the mobile phase were modified stepwise during analysis, this did not overtly interfere with the SERS signals. In addition, the practicability and clinical utility of this approach has also been demonstrated using authentic patients' urine samples. Here,...Continue Reading

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Citations

Sep 30, 2018·Journal of Separation Science·Anna Týčová, Karel Klepárník
Feb 2, 2019·Analytical and Bioanalytical Chemistry·Pengli ZuoQingyou Liu
Sep 10, 2020·Analytical and Bioanalytical Chemistry·Natalia E MarkinaAlexey V Markin
Mar 5, 2019·Metabolomics : Official Journal of the Metabolomic Society·Oliver A H Jones
Jun 21, 2020·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Xin JiangBing Zhao
Jun 4, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Swati TanwarIshan Barman
May 27, 2021·Biosensors & Bioelectronics·Jun Jie OngAbdul W Basit
Apr 8, 2021·Annual Review of Analytical Chemistry·Cassio LimaRoyston Goodacre
Dec 12, 2018·ACS Sensors·Onur DurucanAnja Boisen

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