Quantitative PCR analysis of house dust can reveal abnormal mold conditions

Journal of Environmental Monitoring : JEM
Teija MeklinStephen J Vesper

Abstract

Indoor mold concentrations were measured in the dust of moldy homes (MH) and reference homes (RH) by quantitative PCR (QPCR) assays for 82 species or related groups of species (assay groups). About 70% of the species and groups were never or only rarely detected. The ratios (MH geometric mean : RH geometric mean) for 6 commonly detected species (Aspergillus ochraceus, A. penicillioides, A. unguis, A. versicolor, Eurotium group, and Cladosporium sphaerospermum) were >1 (Group I). Logistic regression analysis of the sum of the logs of the concentrations of Group I species resulted in a 95% probability for separating MH from RH. These results suggest that it may be possible to evaluate whether a home has an abnormal mold condition by quantifying a limited number of mold species in a dust sample. Also, four common species of Aspergillus were quantified by standard culturing procedures and their concentrations compared to QPCR results. Culturing underestimated the concentrations of these four species by 2 to 3 orders of magnitude compared to QPCR.

References

Mar 15, 2001·Journal of Exposure Analysis and Environmental Epidemiology·J D RoeL J Wymer
Mar 2, 2002·Journal of Environmental Monitoring : JEM·Mika ToivolaAino Nevalainen
May 29, 2002·Journal of Microbiological Methods·Richard A HauglandStephen J Vesper
Mar 7, 2003·Applied and Environmental Microbiology·Nichole E BrinkmanStephen J Vesper
Mar 30, 2004·Systematic and Applied Microbiology·Richard A HauglandStephen J Vesper

❮ Previous
Next ❯

Citations

Sep 1, 1984·Environmental Monitoring and Assessment·B S Ausmus
Sep 19, 2009·Archives of Environmental Contamination and Toxicology·Ronald W HardyGregory Möller
Jan 12, 2011·Archives of Environmental Contamination and Toxicology·Michael A MallinEd Wirth
Dec 25, 2010·Applied Microbiology and Biotechnology·Andrea FranzettiGiuseppina Bestetti
Dec 18, 2013·Environmental Monitoring and Assessment·Ademola O OlaniranBalakrishna Pillay
Jun 19, 2009·Journal of Urban Health : Bulletin of the New York Academy of Medicine·Stephen VesperGary Dewalt
Feb 24, 2009·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Yulia Y IossifovaGrace LeMasters
Oct 31, 2006·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Seung-Hyun ChoDavid Bernstein
Oct 13, 2006·Journal of Exposure Science & Environmental Epidemiology·Stephen J VesperTiina Reponen
Apr 6, 2006·Journal of Occupational and Environmental Hygiene·R Todd NiemeierSergey A Grinshpun
May 16, 2007·Journal of Occupational and Environmental Hygiene·H Kenneth DillonJ David Miller
Aug 23, 2008·Journal of Occupational and Environmental Hygiene·Janet MacherCarol Rao
Sep 10, 2008·Journal of Occupational and Environmental Hygiene·Janet MacherCarol Rao
Nov 6, 2007·Applied and Environmental Microbiology·M PitkärantaH Rintala
Mar 7, 2006·Journal of Clinical Microbiology·P R BadenochR A D Mills
Nov 10, 2012·BMC Microbiology·Cindy M LiuLance B Price
Jun 26, 2007·Environmental Health Perspectives·Clifford S MitchellMeryl H Karol
Feb 21, 2009·Environmental Science and Pollution Research International·Mariana NeamtuLuiz F De Alencastro
Aug 5, 2011·Environmental Monitoring and Assessment·Marli GerenuttiMário Sergio Galhiane
Aug 20, 2010·Analytical and Bioanalytical Chemistry·Sarka BidmanovaZbynek Prokop
Jul 5, 2007·Biotechnology Letters·Gangming XuYanchun Yan
May 31, 2001·Environmental Toxicology and Pharmacology·S DekkersM A.J. Rennen
Sep 23, 2006·Environmental Management·Ching-Ho ChenHorng-Guang Leu
Mar 12, 2011·Environmental Monitoring and Assessment·Q D LamN Fohrer
Feb 23, 2011·Environmental Science and Pollution Research International·Samuel ObiriZita Naangmenyele
Dec 31, 2015·Microbiome·Julian GordonJack A Gilbert
Mar 24, 2007·Journal of Environmental Management·Delia Teresa Sponza, Canan Cigal
Jul 22, 2011·Journal of Environmental and Public Health·Stephen VesperWarren Friedman
May 25, 2005·Indoor Air·A Nevalainen, M Seuri
Jun 15, 2006·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Jocelyn M BiaginiJames Lockey
Sep 18, 2009·The Journal of Investigative Dermatology·Jocelyn M Biagini MyersGurjit K Khurana Hershey
Apr 17, 2013·Critical Reviews in Microbiology·Delphine MéheustJean-Pierre Gangneux
Dec 12, 2012·Regulatory Toxicology and Pharmacology : RTP·Patricia EllisKrista Dobo
Jul 21, 2012·Studies in Mycology·K BenschP W Crous
Jun 7, 2014·Frontiers in Microbiology·Jörg EttenauerKatja Sterflinger
Apr 19, 2008·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Marian D GoebesLynn M Hildemann

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.