Quantitative phase spectroscopy.

Biomedical Optics Express
Matthew RinehartAdam Wax

Abstract

Quantitative phase spectroscopy is presented as a novel method of measuring the wavelength-dependent refractive index of microscopic volumes. Light from a broadband source is filtered to an ~5 nm bandwidth and rapidly tuned across the visible spectrum in 1 nm increments by an acousto-optic tunable filter (AOTF). Quantitative phase images of semitransparent samples are recovered at each wavelength using off-axis interferometry and are processed to recover relative and absolute dispersion measurements. We demonstrate the utility of this approach by (i) spectrally averaging phase images to reduce coherent noise, (ii) measuring absorptive and dispersive features in microspheres, and (iii) quantifying bulk hemoglobin concentrations by absolute refractive index measurements. Considerations of using low coherence illumination and the extension of spectral techniques in quantitative phase measurements are discussed.

References

Jun 1, 1957·Journal of the Optical Society of America·R BARER
May 6, 2006·Journal of Biomedical Optics·Michael A ChomaJoseph A Izatt
Nov 30, 2010·Biomedical Optics Express·Dan FuMichael Feld
Apr 20, 2011·Optics Express·Zahid YaqoobMichael S Feld
Jun 17, 2011·Physics in Medicine and Biology·O ZhernovayaA Douplik
Nov 24, 2011·Optics Express·Zahra MonemhaghdoustChristophe Moser
Dec 6, 2011·Optics Letters·Francisco E RoblesAdam Wax
Dec 24, 2011·Journal of Biomedical Optics·Matthew T RinehartAdam Wax

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Citations

Sep 20, 2012·Applied Physics Letters·Niyom LueZahid Yaqoob
May 15, 2013·Annual Review of Biomedical Engineering·P MarquetP J Magistretti
Feb 13, 2016·Optics Letters·Shichao ChenYizheng Zhu
Feb 26, 2016·Optics Express·JaeHwang JungYongKeun Park
Apr 2, 2014·Optics Letters·Matthew T RinehartAdam Wax
Dec 17, 2014·Optics Letters·Kyoohyun KimYongKeun Park
Oct 28, 2015·Biomedical Optics Express·Jonghee YoonYongKeun Park
Oct 17, 2014·Applied Optics·JaeHwang JungYongKeun Park
Dec 8, 2014·Computerized Medical Imaging and Graphics : the Official Journal of the Computerized Medical Imaging Society·Roarke HorstmeyerChanghuei Yang
Dec 22, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Stefano Selci
Oct 17, 2017·Journal of Biomedical Optics·Shilpi AgarwalChandra Shakher
Jan 1, 2019·Journal of Biophotonics·KyeoReh LeeYongKeun Park
Feb 8, 2020·Journal of Biomedical Optics·Manoj KumarOsamu Matoba
May 8, 2019·Biomedical Optics Express·Xiaobo TianRongguang Liang
Dec 8, 2016·Physical Review Letters·Stéphane ClemmenAlexander L Gaeta
Mar 20, 2018·Biomedical Optics Express·Mikhail E KandelGabriel Popescu
Jul 4, 2018·Scientific Reports·Ashkan OjaghiFrancisco E Robles
Jan 8, 2021·Biomedical Optics Express·Konstantin B YushkovVladimir Ya Molchanov
Oct 29, 2017·Methods : a Companion to Methods in Enzymology·Poorya HosseiniPeter T C So
Mar 4, 2017·Biophysical Journal·Will J EldridgeAdam Wax

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