Quantitative phenotypic analysis of yeast deletion mutants using a highly parallel molecular bar-coding strategy

Nature Genetics
D D ShoemakerR W Davis

Abstract

A quantitative and highly parallel method for analysing deletion mutants has been developed to aid in determining the biological function of thousands of newly identified open reading frames (ORFs) in Saccharomyces cerevisiae. This approach uses a PCR targeting strategy to generate large numbers of deletion strains. Each deletion strain is labelled with a unique 20-base tag sequence that can be detected by hybridization to a high-density oligonucleotide array. The tags serve as unique identifiers (molecular bar codes) that allow analysis of large numbers of deletion strains simultaneously through selective growth conditions. Hybridization experiments show that the arrays are specific, sensitive and quantitative. A pilot study with 11 known yeast genes suggests that the method can be extended to include all of the ORFs in the yeast genome, allowing whole genome analysis with a single selective growth condition and a single hybridization.

References

Aug 1, 1990·Trends in Genetics : TIG·C HuxleyI Dunham
Jul 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·V SmithP O Brown
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·D A LashkariT Brennan
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·A C PeaseS P Fodor
Feb 15, 1996·Nature·S G Oliver
Dec 1, 1996·Nature Biotechnology·D J LockhartE L Brown

❮ Previous
Next ❯

Citations

Dec 31, 1997·Genes, Chromosomes & Cancer·S Solinas-ToldoP Lichter
Apr 8, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M S Clark
Apr 8, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D A JohnstonS E Melville
Aug 1, 1997·Electrophoresis·C M Fraser, R D Fleischmann
Jul 2, 2010·Antonie van Leeuwenhoek·J T Trevors, L Masson
May 26, 2005·Journal of Biochemical and Biophysical Methods·Jin K WangZu H Lu
May 26, 2004·Methods : a Companion to Methods in Enzymology·Lili WangCharles S Hoffman
Jul 31, 2003·Pharmacology & Therapeutics·Pamela M CarrollKevin FitzGerald
Oct 9, 2002·Trends in Pharmacological Sciences·John C Game
Sep 23, 1998·Trends in Biotechnology·S G OliverF Baganz
Jul 24, 1998·Trends in Biotechnology·M SchenaR W Davis
Jan 17, 2002·Journal of Biotechnology·Rashmi KorkeWei Shou Hu
Jan 28, 2003·Trends in Genetics : TIG·Patrick F Chinnery
Feb 1, 1997·Trends in Genetics : TIG·W Richards
May 14, 1999·Parasitology Today·K Tetteh
Feb 13, 2001·Current Opinion in Biotechnology·S V Tillib, A D Mirzabekov
Feb 13, 2001·Current Opinion in Biotechnology·S Vidan, M Snyder
Feb 5, 2003·Current Opinion in Biotechnology·Mats GullbergUlf Landegren
Oct 29, 2003·Current Opinion in Biotechnology·Ryan T Gill
Jan 13, 1998·Current Opinion in Biotechnology·L M Gelbert, R E Gregg
Mar 20, 1999·Genetic Analysis : Biomolecular Engineering·R J SapolskyJ B Fan
May 14, 1999·Trends in Plant Science·T MaesT Gerats
Aug 6, 1998·Current Opinion in Chemical Biology·S C Case-GreenE M Southern
Jun 14, 2000·Current Opinion in Microbiology·P S CoelhoM Snyder
Feb 7, 2001·Current Opinion in Microbiology·R A ClaytonC M Fraser
Mar 6, 1999·Current Opinion in Microbiology·C M TangE R Moxon
Dec 15, 2004·Drug Discovery Today·Michael J Taussig, Ulf Landegren
Nov 30, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Lars M Steinmetz, Adam M Deutschbauer
Nov 30, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Peter J Oefner
Nov 4, 2008·Journal of Agricultural and Food Chemistry·Patrick P McCue, James M Phang
Nov 11, 1998·Nature Medicine·D J Lockhart
Jul 20, 2010·Nature Biotechnology·Joseph R WarnerRyan T Gill
Feb 3, 1998·Nature Biotechnology·G Ramsay
Jan 7, 1998·Nature Biotechnology·L WodickaD J Lockhart
Dec 17, 2011·Nature Chemical Biology·Terry RoemerCorey Nislow
Feb 14, 1998·Nature Genetics·J G HaciaF S Collins
Apr 1, 1997·Nature Genetics·S Fields
Dec 1, 1996·Nature Genetics
May 28, 2005·Nature Genetics·Ann-Christine Syvänen
Jul 25, 2006·Nature Methods·Sarah E PierceGuri Giaever
Nov 14, 2006·Nature Methods·Michael D LynchRyan T Gill
Aug 20, 2005·Nature Reviews. Cancer·William G Kaelin
Feb 18, 2006·Nature Reviews. Genetics·Jörg D Hoheisel

❮ Previous
Next ❯

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