Quantitative phenotyping of inflammatory bowel disease in the IL-10-deficient mouse by use of noninvasive magnetic resonance imaging

Inflammatory Bowel Diseases
Svenja MichaelAndré Bleich

Abstract

Although magnetic resonance imaging (MRI) is an increasingly used diagnostic tool in the assessment of inflammatory bowel disease (IBD) in humans, diagnosis and quantitation of intestinal inflammation in animal models of IBD still depends on ex vivo techniques. The aim of this study was to evaluate whether high-field MRI is suitable for the quantitative phenotyping of gut inflammation in a dextran sulfate sodium (DSS)-triggered interleukin (IL)10-deficient (IL-10(-/-)) mouse model of IBD, especially in longitudinal studies. Using colitis-susceptible and -resistant backgrounds, MRI and ex vivo analyses were applied to characterize this specific model, differentiating disease severity and time-dependent alterations. Colon wall thickness, cecum wall tissue intensity, spleen, and mesenteric lymph node (MLN) volumes were evaluated 1, 2, 4, and 12 weeks after disease onset by T2-weighted MRI. Ex vivo parameters included histology, spleen, and MLN weight and analysis of cytokine expression. MRI and ex vivo determined parameters correlated well, revealing a mouse strain-specific colitis development over time with characteristics typical for the DSS model in the initial and for the IL-10(-/-) model in the chronic phase. To evaluate the ...Continue Reading

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Citations

Jul 8, 2014·PloS One·Chloé MelchiorCéline Savoye-Collet
Jul 5, 2015·Applied Microbiology and Biotechnology·Mariana L PalmaBruno Douradinha
Sep 10, 2013·Bioengineered·Bruno DouradinhaErnesto T A Marques
Nov 1, 2016·Journal of Crohn's & Colitis·Alexandra GerstgrasserIsabelle Frey-Wagner
Jul 30, 2014·Inflammatory Bowel Diseases·Daria GusevaEvgeni G Ponimaskin
Feb 12, 2019·Mucosal Immunology·Annika WyssBenjamin Misselwitz
Sep 5, 2014·Proceedings of the National Academy of Sciences of the United States of America·Kyle L FlanniganJohn L Wallace
Nov 14, 2019·Laboratory Animals·Lydia M KeublerAndré Bleich
Jan 27, 2016·World Journal of Gastroenterology : WJG·Markus BrücknerDominik Bettenworth

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