Quantitative Phosphoproteomics Reveals Cell Alignment and Mitochondrial Length Change under Cyclic Stretching in Lung Cells.

International Journal of Molecular Sciences
Wei-Hsuan WangHsueh-Fen Juan

Abstract

Lung cancer is a leading cause of death. Most previous studies have been based on traditional cell-culturing methods. However, lung cells are periodically subjected to mechanical forces during breathing. Understanding the mechanisms underlying the cyclic stretching induced in lung cells may be important for lung cancer therapy. Here, we applied cyclic stretching to stimulate the continual contraction that is present under physiological conditions in lung cells. We first uncovered the stretching-induced phosphoproteome in lung cancer cell line A549 and fibroblast cell line IMR-90. We identified 2048 and 2604 phosphosites corresponding to 837 and 1008 phosphoproteins in A549 and IMR-90, respectively. Furthermore, we combined our phosphoproteomics and public gene expression data to identify the biological functions in response to cyclic stretching. Interestingly, cytoskeletal and mitochondrial reorganization were enriched. We further used cell imaging analysis to validate the profiling results and found that this physical force changed cell alignment and mitochondrial length. This study not only reveals the molecular mechanism of cyclic stretching but also provides evidence that cell stretching causes cellular rearrangement and mi...Continue Reading

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Datasets Mentioned

BETA
PXD313200
GSE15411
GSE80161
PXD012010

Methods Mentioned

BETA
phosphoproteomic profiling
protein modification
Protein Assay

Software Mentioned

Mfuzz
ImageJ
R package “ limma ”
MaxQuant
Zeiss
Reduce and Visualize Gene Ontology ( REVIGO )
Icy
Andromeda
Zen
DAVID

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