Quantitative plasma determination of a novel antiretroviral derivative of zidovudine by solid-phase extraction and high-performance liquid chromatography

Analytical and Bioanalytical Chemistry
Mario A QuevedoMargarita C Briñón

Abstract

A solid-phase extraction methodology, followed by high-performance liquid chromatography (HPLC) quantification with UV absorbance detection (lambda = 267 nm), was developed in order to study the stability of 3'-azido-3'-deoxy-5'-O-isonicotinoylthymidine (AZT-Iso), a novel derivative of the antiretroviral AZT, in different matrixes. The half-lives (t1/2) for AZT-Iso were 1.19, 1.13 and 0.30 h for human, rat and rabbit plasma, respectively, and 14.91 and 25.49 h for potassium phosphate buffer (pH 7.4) and human serum albumin solution, respectively. The HPLC method proved to be selective, sensitive and accurate. Good recovery, linearity and precision were achieved using p-fluorophenol as an internal standard. The validity of this method was tested using synthetic mixtures of the intact drug with its decomposition products. In conclusion, the method presented is applicable to in vivo pharmacokinetics studies of AZT-Iso in rats.

References

Apr 20, 2000·Antiviral Research·G AndreiE De Clercq
Nov 2, 2001·Biochemical and Biophysical Research Communications·M A QuevedoM C Briñón
Apr 24, 2003·Nucleosides, Nucleotides & Nucleic Acids·Maria T BaumgartnerMargarita C Briñón

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Citations

May 28, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Amir Abbas Rafati, Ahmadreza Afraz
May 5, 2011·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Mario A QuevedoMargarita C Briñón
Jun 25, 2009·Antiviral Research·Mario Alfredo Quevedo, Margarita Cristina Briñón
Jul 7, 2011·Journal of Young Pharmacists : JYP·A ChatterjeeY S Thakur
May 26, 2010·Pharmaceutical Development and Technology·Arkendu ChatterjeeAmlan Gupta

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