Quantitative Profiling of Combinational K27/K36 Modifications on Histone H3 Variants in Mouse Organs

Journal of Proteome Research
Yanyan YuWei Yi

Abstract

The coexisting post-translational modifications (PTMs) on histone H3 N-terminal tails were known to crosstalk between each other, indicating their interdependency in the epigenetic regulation pathways. H3K36 methylation, an important activating mark, was recently reported to antagonize with PRC2-mediated H3K27 methylation with possible crosstalk mechanism during transcription regulation process. On the basis of our previous studies, we further integrated RP/HILIC liquid chromatography with MRM mass spectrometry to quantify histone PTMs from various mouse organs, especially the combinatorial K27/K36 marks for all three major histone H3 variants. Despite their subtle difference in physicochemical properties, we successfully obtained decent separation and high detection sensitivity for both histone H3.3 specific peptides and histone H3.1/3.2 specific peptides. In addition, the overall abundance of H3.3 can be quantified simultaneously. We applied this method to investigate the pattern of the combinatorial K27/K36 marks for all three major histone H3 variants across five mouse organs. Intriguing distribution differences were observed not only between different H3 variants but also between different organs. Our data shed the new ins...Continue Reading

References

Dec 2, 1999·Methods : a Companion to Methods in Enzymology·D A WhiteB M Turner
Jul 23, 2004·Current Biology : CB·Craig L Peterson, Marc-André Laniel
Nov 4, 2005·The Journal of Biological Chemistry·Sandra B HakeDonald F Hunt
Jul 19, 2006·PLoS Genetics·Siew Loon OoiSteven Henikoff
Oct 21, 2006·Molecular Cell·Alejandra LoyolaGeneviève Almouzni
Feb 27, 2007·Cell·Tony Kouzarides
Jul 24, 2007·Molecular & Cellular Proteomics : MCP·Débora BonenfantJan van Oostrum
Aug 15, 2008·Journal of Proteome Research·Benjamin A GarciaCraig A Mizzen
Jan 18, 2011·The Journal of Biological Chemistry·Wen YuanBing Zhu
Apr 26, 2011·Oncogene·J FüllgrabeB Joseph
Jul 12, 2011·Nucleic Acids Research·Hyunjung KimWoojin An
Sep 14, 2011·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Jakob H Waterborg
Dec 19, 2012·Epigenomics·Wolfgang Fischle
Nov 16, 2013·Cancer Cell·Benjamin T K Yuen, Paul S Knoepfler
Apr 4, 2014·Genes & Development·Christopher M Weber, Steven Henikoff
Apr 5, 2014·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Chang Huang, Bing Zhu
Aug 22, 2014·Development·Benjamin T K YuenPaul S Knoepfler
Aug 29, 2014·Analytical Chemistry·Jun GaoShaolian Zhou
Sep 19, 2014·Biochemical Society Transactions·Michela BorghesanManlio Vinciguerra
Oct 11, 2014·Cell·He HuangYingming Zhao
Oct 25, 2014·F1000prime Reports·Zachary A Gurard-Levin, Geneviève Almouzni

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Citations

Jan 31, 2017·Nature Chemical Biology·Wei QiEn Li
May 31, 2019·Cellular and Molecular Life Sciences : CMLS·Jie LiGang Greg Wang
Jun 24, 2018·Proteomes·Sara El KennaniDelphine Pflieger
Jul 19, 2017·Cellular and Molecular Life Sciences : CMLS·Mei-Ren PanWen-Chun Hung
Jan 31, 2020·Frontiers in Genetics·Mattia ZaghiAlessandro Sessa
Dec 2, 2020·Developmental Neurobiology·Cheng ZhangFengyuan Che
Mar 15, 2020·Progress in Neuro-psychopharmacology & Biological Psychiatry·Wenxin LiuDonghong Cui
Mar 12, 2021·Developmental Biology·Bridget T Jacques-FrickeLaura S Gammill
Sep 10, 2021·Genesis : the Journal of Genetics and Development·Julaine Roffers-AgarwalLaura S Gammill
Oct 31, 2021·Clinical Epigenetics·Chu XiaoJie He

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