Quantitative Profiling of Feruloylated Arabinoxylan Side-Chains from Graminaceous Cell Walls

Frontiers in Plant Science
Rachel R SchendelMirko Bunzel

Abstract

Graminaceous arabinoxylans are distinguished by decoration with feruloylated monosaccharidic and oligosaccharidic side-chains. Although it is hypothesized that structural complexity and abundance of these feruloylated arabinoxylan side-chains may contribute, among other factors, to resistance of plant cell walls to enzymatic degradation, quantitative profiling approaches for these structural units in plant cell wall materials have not been described yet. Here we report the development and application of a rapid and robust method enabling the quantitative comparison of feruloylated side-chain profiles in cell wall materials following mildly acidic hydrolysis, C18-solid phase extraction (SPE), reduction under aprotic conditions, and liquid chromatography with diode-array detection/mass spectrometry (LC-DAD/MS) separation and detection. The method was applied to the insoluble fiber/cell wall materials isolated from 12 whole grains: wild rice (Zizania aquatica L.), long-grain brown rice (Oryza sativa L.), rye (Secale cereale L.), kamut (Triticum turanicum Jakubz.), wheat (Triticum aestivum L.), spelt (Triticum spelta L.), intermediate wheatgrass (Thinopyrum intermedium), maize (Zea mays L.), popcorn (Zea mays L. var. everta), oat (...Continue Reading

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Citations

Oct 18, 2016·Frontiers in Plant Science·Anne-Laure Chateigner-BoutinLuc Saulnier
Apr 24, 2020·Nature Communications·Dhananjay BeriChristopher D Herring
Jan 24, 2020·Natural Product Reports·Ewelina MnichPeter Ulvskov
Jan 21, 2021·Annual Review of Food Science and Technology·Shang LinAnne S Meyer
Apr 24, 2020·Journal of Agricultural and Food Chemistry·Jie LiuYanbei Wu
May 16, 2018·Journal of Agricultural and Food Chemistry·Catherine LapierreJohn Ralph

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Methods Mentioned

BETA
acylation
NMR

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