Quantitative profiling of mRNA expression of glutathione S-transferase superfamily genes in various tissues of bighead carp (Aristichthys nobilis)

Journal of Biochemical and Molecular Toxicology
Guangyu LiQian Xiong

Abstract

The expression of glutathione S-transferase (GST) is a crucial factor in determining the sensitivity of cells and organs in response to a variety of toxicants. In this study, we cloned the core nucleotide of alpha, kappa, mu, mGST, pi, rho, and theta-like GST genes from bighead carp (Aristichthys nobilis). Their derived amino acid sequences were clustered with other vertebrate GSTs in a phylogenetic tree, and the bighead carp GST sequences have the highest similarity with those from common carp and zebrafish. We quantified the constitutive mRNA transcription of GST isoforms in eight different tissues (liver, kidney, spleen, intestine, muscle, heart, brain, and gill). The information obtained from the present study could be distilled into a few generalized principles: multiple GST isoenzymes were ubiquitously expressed in all tissues; majority of GSTs had high constitutive expression in intestine, liver, and kidney. These findings are in agreement with the roles of these tissues in xenobiotic metabolism. At the same time, some unique findings were detected in the current experiment: (1) higher expression of most GSTs was observed in spleen; (2) the expression of GST pi was highest in almost all the studied tissues except muscle;...Continue Reading

References

Jul 15, 1992·The Science of the Total Environment·D C MuirR J Norstrom
Jul 1, 1990·Toxicology and Applied Pharmacology·S GuptaY C Awasthi
Jun 1, 1997·Environmental Health Perspectives·J Seidegård, G Ekström
Jul 29, 1998·International Journal of Cancer. Journal International Du Cancer·S T SaarikoskiA Hirvonen
Mar 9, 2002·Toxicology and Applied Pharmacology·Philip J SherrattJohn D Hayes
Jun 12, 2003·The Science of the Total Environment·Suwarna PandeySheikh Raisuddin
Jun 20, 2003·Journal of Biochemical and Molecular Toxicology·S A SheweitaW El-Sayed
Aug 21, 2003·Ecotoxicology and Environmental Safety·Iqbal SayeedSheikh Raisuddin
Dec 5, 2003·Nucleic Acids Research·Xiaowei Wang, Brian Seed
Apr 22, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Rachel E ThomsonJohn D Hayes
Dec 22, 2004·The Biochemical Journal·Takafumi KonishiYutaka Tamaru
Apr 12, 2005·Annual Review of Pharmacology and Toxicology·John D HayesIan R Jowsey
Sep 25, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Tamara Raphael KnightCurtis D Klaassen
Oct 4, 2008·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Jin-Hyoung KimJae-Seong Lee
Nov 6, 2008·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Seonock WooHong-Seog Park

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Citations

Jan 10, 2012·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Joana CostaMarta Ferreira
Dec 3, 2014·Aquatic Toxicology·Branka GlisicTvrtko Smital
Jun 18, 2017·Journal of Biochemical and Molecular Toxicology·Maryam S AbunnajaMing-Cheh Liu

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