Quantitative promoter analysis in Physcomitrella patens: a set of plant vectors activating gene expression within three orders of magnitude

BMC Biotechnology
Verena HorstmannEva L Decker

Abstract

In addition to studies of plant gene function and developmental analyses, plant biotechnological use is largely dependent upon transgenic technologies. The moss Physcomitrella patens has become an exciting model system for studying plant molecular processes due to an exceptionally high rate of nuclear gene targeting by homologous recombination compared with other plants. However, its use in transgenic approaches requires expression vectors that incorporate sufficiently strong promoters. To satisfy this requirement, a set of plant expression vectors was constructed and equipped with either heterologous or endogenous promoters. Promoter activity was quantified using the dual-luciferase reporter assay system. The eight different heterologous promoter constructs tested exhibited expression levels spanning three orders of magnitude. Of these, the complete rice actin1 gene promoter showed the highest activity in Physcomitrella, followed by a truncated version of this promoter and three different versions of the cauliflower mosaic virus 35S promoter. In contrast, the Agrobacterium tumefaciens nopaline synthase promoter induced transcription rather weakly. Constructs including promoters commonly used in mammalian expression systems als...Continue Reading

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Citations

Aug 2, 2005·Applied Microbiology and Biotechnology·Andreas WeiseGilbert Gorr
Aug 19, 2007·Bioprocess and Biosystems Engineering·Eva L Decker, Ralf Reski
Dec 6, 2011·Journal of Experimental Botany·Amélie SaumonneauRossitza Atanassova
Oct 3, 2006·Plant Physiology·Adam M TakosAmanda R Walker
Oct 1, 2011·Plant Cell Reports·Eva L Decker, Ralf Reski
Feb 5, 2016·Journal of Experimental Botany·Dong DuanPeter Nick
May 25, 2005·Plant Biology·W FrankR Reski
Jul 30, 2014·Frontiers in Plant Science·Eva L DeckerRalf Reski
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Sep 18, 2007·Current Opinion in Biotechnology·Eva L Decker, Ralf Reski
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Jan 18, 2011·The Plant Journal : for Cell and Molecular Biology·Omar SalehTzahi Arazi
May 27, 2015·Plant Biotechnology Journal·Ralf ReskiEva L Decker
May 26, 2018·Plant Cell Reports·Ralf ReskiHenrik Toft Simonsen
Dec 1, 2018·BMC Plant Biology·Preshobha K PeethambaranMichael Riemann
Jul 19, 2019·The New Phytologist·Mattias ThelanderEva Sundberg
Jan 5, 2021·Frontiers in Plant Science·Lennard L BohlenderEva L Decker

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Datasets Mentioned

BETA
AJ618932
AJ618933

Methods Mentioned

BETA
transgenic
phosphotransferase
transfection
PCRs
PCR

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