Quantitative proteome analysis of temporally resolved phagosomes following uptake via key phagocytic receptors.

Molecular & Cellular Proteomics : MCP
Brian D DillMatthias Trost

Abstract

Macrophages operate at the forefront of innate immunity and their discrimination of foreign versus "self" particles is critical for a number of responses including efficient pathogen killing, antigen presentation, and cytokine induction. In order to efficiently destroy the particles and detect potential threats, macrophages express an array of receptors to sense and phagocytose prey particles. In this study, we accurately quantified a proteomic time-course of isolated phagosomes from murine bone marrow-derived macrophages induced by particles conjugated to seven different ligands representing pathogen-associated molecular patterns, immune opsonins or apoptotic cell markers. We identified a clear functional differentiation over the three timepoints and detected subtle differences between certain ligand-phagosomes, indicating that triggering of receptors through a single ligand type has mild, but distinct, effects on phagosome proteome and function. Moreover, our data shows that uptake of phosphatidylserine-coated beads induces an active repression of NF-κB immune responses upon Toll-like receptor (TLR)-activation by recruitment of anti-inflammatory regulators to the phagosome. This data shows for the first time a systematic time...Continue Reading

References

Dec 29, 1978·Biochemical and Biophysical Research Communications·Y EshdatD Mirelman
Jan 1, 1966·The Journal of Experimental Medicine·A Berken, B Benacerraf
Jan 1, 1983·The Journal of Cell Biology·S S SungS C Silverstein
Mar 21, 1998·The Journal of Biological Chemistry·O ElomaaK Tryggvason
Dec 8, 1998·The Journal of Experimental Medicine·C J KirschningM Rothe
Feb 17, 1999·The Journal of Experimental Medicine·S T QureshiD Malo
Jan 10, 2001·The Journal of Cell Biology·J GarinM Desjardins
Mar 30, 2001·The Journal of Biological Chemistry·J F DermineM Desjardins
Dec 26, 2001·The Journal of Biological Chemistry·Guolong Zhang, Sankar Ghosh
Jan 26, 2002·Human Molecular Genetics·Bart van De SluisCisca Wijmenga
Feb 21, 2002·Molecular Biology of the Cell·Maria DiakonovaJoel A Swanson
May 10, 2002·Nature·Rikinari HanayamaShigekazu Nagata
Jan 24, 2004·The Journal of Biological Chemistry·Kensuke OikawaMasatoshi Tateno
Feb 12, 2004·Bioinformatics·M J L de HoonS Miyano
Mar 9, 2004·Molecular Biology of the Cell·Jagath R JunutulaRichard H Scheller
May 15, 2004·Science·J Magarian Blander, Ruslan Medzhitov
Jul 13, 2004·Trends in Cell Biology·Gareth Griffiths
Mar 16, 2005·Journal of Leukocyte Biology·Véronique Le CabecIsabelle Maridonneau-Parini
Apr 1, 2005·The Journal of Biological Chemistry·Ezra BursteinColin S Duckett
Oct 11, 2005·Annual Review of Cell and Developmental Biology·Isabelle Jutras, Michel Desjardins
Nov 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Rikinari Hanayama, Shigekazu Nagata
Dec 31, 2005·Nucleic Acids Research·Frank DesiereRuedi Aebersold
Apr 1, 2006·The Biochemical Journal·Prim de BieLeo W J Klomp
Apr 18, 2006·Cell·Michael S KostelanskyJames H Hurley
Aug 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Lars-Peter ErwigPeter M Henson
Sep 21, 2006·Nature Immunology·J Magarian Blander, Ruslan Medzhitov
Dec 2, 2006·Experimental Cell Research·Takayuki KuraishiYoshinobu Nakanishi
Feb 17, 2007·Nature Immunology·David G Russell, Robin M Yates
Mar 16, 2007·Cell Cycle·Gabriel N Maine, Ezra Burstein
Apr 25, 2007·The Journal of Cell Biology·Melanie M BrinkmannYou-Me Kim
Oct 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Camila M PeresMarc Peters-Golden

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Citations

Mar 18, 2016·Immunity·Siamon Gordon
Mar 30, 2017·The Journal of Experimental Medicine·David C ThomasKenneth G C Smith
Nov 9, 2017·Frontiers in Immunology·Eileen Uribe-Querol, Carlos Rosales
May 24, 2018·Physiological Reviews·Ilse DingjanGeert van den Bogaart
Mar 7, 2018·Molecular & Cellular Proteomics : MCP·Hella SchmidtAxel A Brakhage
Feb 17, 2017·Journal of Cell Science·Ilse DingjanGeert van den Bogaart
May 3, 2018·American Journal of Physiology. Cell Physiology·Spring DavisChanglong Nan
Feb 28, 2019·Molecular & Cellular Proteomics : MCP·Anne-Marie PauwelsEik Hoffmann
Oct 17, 2015·The EMBO Journal·Yu-Chiang LaiMiratul Mk Muqit
Apr 28, 2019·The EMBO Journal·Manman GuoMatthias Trost
May 24, 2018·The EMBO Journal·Anetta HärtlovaMaximiliano G Gutierrez
Jul 30, 2017·Biology of the Cell·Gholamreza Fazeli, Ann Marie Wehman
Aug 7, 2020·The FEBS Journal·Roni Levin-Konigsberg, Adriana R Mantegazza
Jul 25, 2018·Nature Microbiology·Katharina TrunkSarah J Coulthurst
May 1, 2019·Molecular & Cellular Proteomics : MCP·Anne-Marie PauwelsEik Hoffmann
Jul 31, 2020·Current Opinion in Cell Biology·Kevin P Bohannon, Phyllis I Hanson
Mar 16, 2021·Frontiers in Immunology·Jenny A Nguyen, Robin M Yates
Sep 10, 2020·ACS Nano·Bill LingMikhail G Shapiro

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Methods Mentioned

BETA
Flow cytometry
acetylation
PCA
flow
dot blots

Software Mentioned

Cytoscape
Maxquant
PDL scripts
Perl
Rapid Scan
Flowjo
PeptideAtlas
Skyline
STRING
custom

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