Quantitative proteomic analyses reveal that energy metabolism and protein biosynthesis reinitiation are responsible for the initiation of bolting induced by high temperature in lettuce (Lactuca sativa L.).

BMC Genomics
Jing-hong HaoShuang-xi Fan

Abstract

Lettuce (Lactuca sativa L.), one of the most economically important leaf vegetables, exhibits early bolting under high-temperature conditions. Early bolting leads to loss of commodity value and edibility, leading to considerable loss and waste of resources. However, the initiation and molecular mechanism underlying early bolting induced by high temperature remain largely elusive. In order to better understand this phenomenon, we defined the lettuce bolting starting period, and the high temperature (33 °C) and controlled temperature (20 °C) induced bolting starting phase of proteomics is analyzed, based on the iTRAQ-based proteomics, phenotypic measurement, and biological validation by RT-qPCR. Morphological and microscopic observation showed that the initiation of bolting occurred 8 days after high-temperature treatment. Fructose accumulated rapidly after high-temperature treatment. During initiation of bolting, of the 3305 identified proteins, a total of 93 proteins exhibited differential abundances, 38 of which were upregulated and 55 downregulated. Approximately 38% of the proteins were involved in metabolic pathways and were clustered mainly in energy metabolism and protein synthesis. Furthermore, some proteins involved in ...Continue Reading

References

May 3, 2000·International Journal of Food Microbiology·L Plourde-OwobiJ François
Dec 29, 2000·Nature·M SalanoubatUNKNOWN Kazusa DNA Research Institute
Sep 21, 2004·Annual Review of Plant Biology·Yoshibumi Komeda
Aug 13, 2005·The International Journal of Developmental Biology·François Parcy
Jul 15, 2006·PLoS Genetics·Sureshkumar BalasubramanianDetlef Weigel
May 29, 2007·Nucleic Acids Research·Yuki MoriyaMinoru Kanehisa
Dec 7, 2007·Mitochondrion·Jenny CarlssonKristina Glimelius
Apr 21, 2009·Nature Methods·Jacek R WiśniewskiMatthias Mann
Mar 1, 2003·Expert Review of Neurotherapeutics·Joshua M Levine, Scott E Kasner
May 26, 2011·Cellular and Molecular Life Sciences : CMLS·Anusha Srikanth, Markus Schmid
Dec 18, 2015·The Plant Pathology Journal·Chang-Jin Park, Young-Su Seo
Dec 27, 2016·Frontiers in Plant Science·Yingyan HanXiaolan Zhang
May 18, 2018·The Plant Journal : for Cell and Molecular Biology·Zijing ChenQian Wang
Aug 21, 2018·Bioscience, Biotechnology, and Biochemistry·Ginga ShimakawaChikahiro Miyake

❮ Previous
Next ❯

Software Mentioned

CFX manager
Trinotate
MASCOT
Proteome Discoverer
ClueGO
Microsoft Office PowerPoint
SPSS
BLAST
Origin Pro
Blast2GO

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.