Quantitative proteomic analysis of B cell lipid rafts reveals that ezrin regulates antigen receptor-mediated lipid raft dynamics

Nature Immunology
Neetu GuptaAnthony L DeFranco

Abstract

Ligation of the B cell antigen receptor (BCR) with antigen induces lipid raft coalescence, a process that occurs after crosslinking of a variety of signaling receptors and is thought to potentiate cellular activation. To investigate lipid raft dynamics during BCR signaling, we quantitatively analyzed the B cell lipid raft proteome. BCR engagement induced dissociation of the adaptor protein ezrin from lipid rafts as well as threonine dephosphorylation of ezrin and its concomitant detachment from actin, indicating a transient uncoupling of lipid rafts from the actin cytoskeleton. Expression of constitutively active ezrin chimeras inhibited the BCR-induced coalescence of lipid rafts. Our data demonstrate that the release of ezrin from lipid rafts acts as a critical trigger that regulates lipid raft dynamics during BCR signaling.

References

Aug 1, 1996·Current Opinion in Cell Biology·A Kusumi, Y Sako
Jun 1, 1997·Current Opinion in Immunology·A L DeFranco
Jan 19, 1999·Annual Review of Cell and Developmental Biology·D A Brown, E London
Feb 27, 1999·Current Opinion in Cell Biology·A Bretscher
Aug 17, 1999·Current Opinion in Cell Biology·T V Kurzchalia, R G Parton
Dec 10, 1999·The Journal of Experimental Medicine·P C ChengS K Pierce
Jul 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·R J PetrieJ P Deans
Nov 9, 2000·Biology of the Cell·S Louvet-Vallée
Jan 13, 2001·Current Opinion in Immunology·K Siemasko, M R Clark
Mar 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·P C ChengS K Pierce
Jan 5, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Katsuhiko ItohTakashi Saito
Mar 26, 2002·Nature Reviews. Immunology·Susan K Pierce
Jun 12, 2002·The Journal of Cell Biology·Takahiro FujiwaraAkihiro Kusumi
Oct 22, 2002·Immunity·Angela StoddartFrances M Brodsky
Nov 26, 2002·Immunological Reviews·Patrick CullinanJanis K Burkhardt
Nov 25, 2003·Analytical Chemistry·Alexey I NesvizhskiiRuedi Aebersold
Feb 6, 2004·Nature Immunology·Sophie FaureJérôme Delon
Nov 6, 2004·Nature Biotechnology·Patrick G A PedrioliRuedi Aebersold
Jul 13, 2005·The Journal of Cell Biology·Joseph LinAndrey S Shaw
Aug 11, 2005·Cellular and Molecular Life Sciences : CMLS·M F LanghorstC A O Stuermer
Dec 17, 2005·Immunity·Karen SommerDavid J Rawlings

❮ Previous
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Citations

Apr 25, 2007·Apoptosis : an International Journal on Programmed Cell Death·Walter MalorniMaurizio Sorice
Jan 12, 2011·Immunology and Cell Biology·Robynn V SchillaceDaniel W Carr
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Yael SagiShoshana Levy
Feb 12, 2009·The Journal of Biological Chemistry·Laura MontaniMartin E Schwab
Apr 7, 2009·Molecular & Cellular Proteomics : MCP·Robert S BoydKelvin Cain
Jun 16, 2009·Molecular & Cellular Proteomics : MCP·Thomas StanislasFrançoise Simon-Plas
Apr 13, 2011·The Journal of Experimental Medicine·Bebhinn TreanorFacundo D Batista
Jun 29, 2007·Molecular Biology of the Cell·Delphine Le RouxAna-Maria Lennon-Duménil
Jan 16, 2009·Biology of Reproduction·Patricia V MirandaPablo E Visconti
Nov 5, 2010·Cold Spring Harbor Perspectives in Biology·Naomi E Harwood, Facundo D Batista
Mar 3, 2010·Annual Review of Immunology·Naomi E Harwood, Facundo D Batista
Apr 6, 2013·Biology Direct·Katarína VaškovičováJan Brábek
Jan 13, 2009·Journal of Lipid Research·Yu Zi ZhengLeonard J Foster
Oct 29, 2008·Journal of Lipid Research·Linda J Pike
Jul 7, 2009·Journal of Lipid Research·Yu Zi Zheng, Leonard J Foster
Nov 10, 2013·PloS One·Johanna SalonenMauno Vihinen
Nov 12, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ioana CarceaDeanna L Benson
May 15, 2010·Future Oncology·Faustino Mollinedo, Consuelo Gajate
Jan 23, 2014·Nature Immunology·Felix Meissner, Matthias Mann
Dec 25, 2012·Molecular Biology of the Cell·Juan F ArandaJaime Millán
Nov 12, 2013·Oncogene·M Hrdinka, V Horejsi
Sep 16, 2011·Leukemia & Lymphoma·Tri-Hung NguyenSrinivas Shankara
Mar 29, 2007·The Journal of Cell Biology·Fulvia VascottoAna-Maria Lennon-Duménil
Oct 6, 2010·FEBS Letters·Jianying Yang, Michael Reth
Jan 21, 2009·The Journal of Cell Biology·Yuntao S MaoHelen L Yin
Oct 6, 2007·Expert Opinion on Drug Metabolism & Toxicology·Ben C CollinsStephen R Pennington
Sep 26, 2015·Proteomics·María GatoEnrique Santamaría
May 28, 2011·Trends in Biochemical Sciences·Jeffrey N SavasJohn R Yates
May 4, 2010·Current Opinion in Immunology·Bebhinn Treanor, Facundo D Batista
Apr 22, 2009·Prostaglandins & Other Lipid Mediators·Jeffrey L Dupree, Anthony D Pomicter
Oct 18, 2008·Journal of Proteomics·Yu Zi Zheng, Leonard J Foster

❮ Previous
Next ❯

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