Quantitative proteomic analysis of MDCK cell adhesion.

Molecular Omics
Xuanqing YeZhenbin Liu

Abstract

MDCK cells are a key reagent in modern vaccine production. As MDCK cells are normally adherent, creation of suspension cells for vaccine production using genetic engineering approaches is highly desirable. However, little is known regarding the mechanisms and effectors underlying MDCK cell adhesion. In this study, we performed a comparative analysis of whole protein levels between MDCK adhesion and suspension cells using an iTRAQ-based (isobaric tags for relative and absolute quantitation) proteomics approach. We found that expression of several proteins involved in cell adhesion exhibit reduced expression in suspension cells, including at the mRNA level. Proteins whose expression was reduced in suspension cells include cadherin 1 (CDH1), catenin beta-1 (CTNNB1), and catenin alpha-1 (CTNNA1), which are involved in intercellular adhesion; junction plakoglobin (JUP), desmoplakin (DSP), and desmoglein 3 (DSG3), which are desmosome components; and transglutaminase 2 (TGM2) and alpha-actinin-1 (ACTN1), which regulate the adhesion between cells and the extracellular matrix. A functional verification experiment showed that inhibition of E-cadherin significantly reduced intercellular adhesion of MDCK cells. E-Cadherin did not significa...Continue Reading

References

Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M TaubG Sato
Feb 1, 1994·The Journal of Cell Biology·S M Frisch, H Francis
Apr 1, 1994·The Journal of Cell Biology·S V LitvinovS O Warnaar
Nov 18, 1998·Current Opinion in Cell Biology·A Ben-Ze'ev, B Geiger
Jan 9, 2001·Nature Cell Biology·C E Turner
Jul 6, 2004·Journal of Virological Methods·R YouilD DiStefano
Jul 17, 2004·Annual Review of Biomedical Engineering·Christopher S ChenJoe Tien
Jan 22, 2005·Applied Microbiology and Biotechnology·N Bardiya, J H Bae
Dec 27, 2005·Current Opinion in Genetics & Development·Felix H BrembeckWalter Birchmeier
Jul 24, 2007·Nature Reviews. Molecular Cell Biology·Thomas Lecuit, Pierre-François Lenne
Sep 15, 2007·Biochimica Et Biophysica Acta·David Garrod, Martyn Chidgey
Aug 30, 2008·Cellular and Molecular Life Sciences : CMLS·F van Roy, G Berx
Oct 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Leonie F WaandersMatthias Mann
Dec 4, 2009·Journal of Cell Science·Bhushan V DesaiKathleen J Green
May 13, 2010·Journal of Cell Science·Micha NethePeter L Hordijk
Jan 1, 2007·The Journal of Investigative Dermatology·Gemma HopkinsDavid R Garrod
Nov 16, 2012·Nature Reviews. Molecular Cell Biology·Alan Rick Horwitz
Mar 15, 2013·Developmental Cell·Haguy WolfensonBenjamin Geiger
Jun 8, 2013·Science·Charlène Guillot, Thomas Lecuit
Nov 7, 2013·Cell Adhesion & Migration·Evelyn Png, Louis Tong
Jan 21, 2014·Nature Reviews. Cancer·Frans van Roy
Mar 13, 2014·Journal of Cell Science·Molly LowndesW James Nelson
Nov 29, 2014·The Journal of Investigative Dermatology·Kevin J HamillJonathan C R Jones
Jan 1, 2007·The Journal of Investigative Dermatology·Gemma HopkinsDavid R Garrod
Aug 16, 2016·International Journal of Molecular Sciences·Ilaria Prada, Jacopo Meldolesi
Mar 12, 2018·Current Opinion in Cell Biology·Masatoshi Takeichi
Aug 19, 2018·Scientific Reports·Evelyn PngLouis Tong

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Methods Mentioned

BETA
PCR
electrophoresis
fluorescence microscopy

Software Mentioned

GraphPad Prism
MaxQuant
MaxQuant engine

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Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

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