Quantitative Proteomic Analysis of Peripheral Blood Mononuclear Cells in Ankylosing Spondylitis by iTRAQ

Clinical and Translational Science
Anji CaiYong Dai

Abstract

This study was designed to identify and quantify the different proteins expression levels in ankylosing spondylitis (AS) and to explore the pathogenesis of AS. We performed isobaric tags for relative and absolute quantitation (iTRAQ) coupled with multiple chromatographic fractionation and tandem mass spectrometry to detect the proteins profiling in peripheral blood mononuclear cells (PBMCs) from AS patients and healthy controls. Mascot software and the International Protein Index and the Gene Ontology (GO) database were used to conduct the bioinformatics analysis. The differentially expressed proteins were validated by enzyme-linked immunosorbent assay (ELISA). A total of 1,232 proteins were identified by iTRAQ, of which 183 showed differential expression and 18 differentially expressed proteins were acute phase reactants. Upon mapping of the differentially expressed proteins to GO database, we found four differentially expressed proteins involved in the biological process of cell killing, including up-regulated cathepsin G (CTSG), neutrophil defensin3 (DEFA3), protein tyrosine phosphatase receptor type C (PTPRC), and down-regulated peroxiredoxin-1(PRDX1),which were consistent with the verified results of ELISA. Our proteomic a...Continue Reading

References

Jan 1, 1995·Cellular Immunology·T Hase-Yamazaki, Y Aoki
Apr 16, 1998·Immunology·T Yamazaki, Y Aoki
Jan 9, 2003·Trends in Biochemical Sciences·Zachary A WoodLeslie B Poole
Jun 7, 2005·International Immunology·Niva Azuz-LiebermanOfer Mandelboim
Jun 17, 2006·Best Practice & Research. Clinical Rheumatology·Judith A SmithRobert A Colbert
Aug 11, 2006·Rheumatology International·Hiroyuki TamiyaSaburo Sone
Apr 17, 2007·Biochemical and Biophysical Research Communications·Jing LiuYuan Ou
Jun 6, 2009·Current Opinion in Rheumatology·Matthew A Brown
Dec 19, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jonah R RiddellSandra O Gollnick
Nov 3, 2010·Best Practice & Research. Clinical Rheumatology·Kurt de Vlam
Dec 15, 2010·BMC Musculoskeletal Disorders·Xiaotian ChangYazhou Cui
Mar 16, 2011·Annals of the Rheumatic Diseases·Nathan VastesaegerJürgen Braun
Jun 28, 2011·Digestive Diseases and Sciences·Cathrine JespersgaardPaal Skytt Andersen
Apr 20, 2012·Nature Immunology·Inmoo Rhee, André Veillette
Feb 19, 2013·The Journal of Rheumatology·Nadine Zeboulon-KtorzaThomas Hanslik
May 20, 2014·Clinical Rheumatology·John D Reveille

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Citations

Jun 29, 2018·Current Rheumatology Reports·Darren D O'RiellyProton Rahman
Jul 2, 2020·Nature Reviews. Rheumatology·Matthew A BrownKim-Anh Lê Cao

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