Quantitative proteomic analysis of post-translational modifications of human histones.

Molecular & Cellular Proteomics : MCP
Hans Christian BeckOle Nørregaard Jensen

Abstract

Histone proteins are subject to a range of post-transcriptional modifications in living cells. The combinatorial nature of these modifications constitutes the "histone code" that dictates chromatin structure and function during development, growth, differentiation, and homeostasis of cells. Deciphering of the histone code is hampered by the lack of analytical methods for monitoring the combinatorial complexity of reversible multisite modifications of histones, including acetylation and methylation. To address this problem, we used LC-MSMS technology and Virtual Expert Mass Spectrometrist software for qualitative and quantitative proteomic analysis of histones extracted from human small cell lung cancer cells. A total of 32 acetylations, methylations, and ubiquitinations were located in the human histones H2A, H2B, H3, and H4, including seven novel modifications. An LC-MSMS-based method was applied in a quantitative proteomic study of the dose-response effect of the histone deacetylase inhibitor (HDACi) PXD101 on histone acetylation in human cell cultures. Triplicate LC-MSMS runs at six different HDACi concentrations demonstrated that PXD101 affects acetylation of histones H2A, H2B, H3, and H4 in a site-specific and dose-depende...Continue Reading

References

Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Jan 19, 2000·Nature·B D Strahl, C D Allis
Aug 3, 2000·Journal of the National Cancer Institute·P A MarksR A Rifkind
Aug 17, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·B M Turner
Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·V G TusherG Chu
Mar 15, 2002·Current Opinion in Genetics & Development·Shelley L Berger
Mar 21, 2002·Nature Reviews. Cancer·P MarksW K Kelly
Jul 18, 2002·Nature Reviews. Drug Discovery·Ricky W Johnstone
Nov 7, 2002·Cell·Bryan M Turner
Nov 7, 2002·Cell·Theodora AgaliotiDimitris Thanos
Nov 30, 2002·Expert Opinion on Investigational Drugs·William K KellyPaul A Marks
Feb 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Huck Hui NgKevin Struhl
Mar 1, 2003·Nature Biotechnology·Matthias Mann, Ole N Jensen
Jul 11, 2003·Nucleic Acids Research·Stuart N PeirsonRussell G Foster
Oct 3, 2003·Nature·Wolfgang FischleC David Allis
Oct 28, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yuzuru Shiio, Robert N Eisenman
Mar 24, 2004·Current Opinion in Chemical Biology·Ole Nørregaard Jensen
Jun 24, 2004·Journal of Cellular Biochemistry·Michael A FreitasMark R Parthun
Aug 26, 2004·Advances in Cancer Research·Paul A MarksWilliam Kevin Kelly
Nov 16, 2004·Biochemical and Biophysical Research Communications·K B GlaserM L Curtin
Mar 3, 2005·Molecular & Cellular Proteomics : MCP·Jennifer Listgarten, Andrew Emili
Jun 14, 2005·Nucleic Acids Research·Yasuo TsunakaKosuke Morikawa

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Citations

Oct 17, 2008·Cellular and Molecular Life Sciences : CMLS·S S NgR J Klose
Aug 24, 2010·Amino Acids·Ry Y Tweedie-Cullen, Isabelle M Mansuy
Apr 24, 2009·Biochemistry·Toyotaka IshibashiJuan Ausió
Aug 25, 2012·Journal of the American Chemical Society·Ian R BothwellMinkui Luo
Jul 6, 2011·Journal of Proteome Research·Pedro Beltran-AlvarezRamon Brugada
Aug 15, 2008·Journal of Proteome Research·Benjamin A GarciaCraig A Mizzen
Sep 22, 2009·Journal of Proteome Research·Mariana D Plazas-MayorcaBenjamin A Garcia
Jun 7, 2013·Nature Structural & Molecular Biology·Philipp Tropberger, Robert Schneider
Apr 20, 2011·The Journal of Biological Chemistry·You FengY George Zheng
Aug 30, 2008·Molecular & Cellular Proteomics : MCP·Junmei ZhangYingming Zhao
Jan 27, 2011·Briefings in Functional Genomics·Annalisa Izzo, Robert Schneider
Feb 24, 2009·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Anita A ThambirajahJuan Ausió
Oct 16, 2007·Journal of Translational Medicine·Michael T BuckleyIman Osman
Sep 22, 2011·PloS One·Mariana P TorrenteBenjamin A Garcia
Aug 20, 2014·Drugs·Raewyn M Poole
Apr 30, 2008·FEBS Letters·Laura Trinkle-Mulcahy, Angus I Lamond
Oct 9, 2009·Expert Review of Proteomics·Martin Kussmann
Feb 19, 2011·Expert Review of Proteomics·Sonja ReilandJeroen Krijgsveld
Sep 25, 2015·Expert Review of Proteomics·Özlem ÖnderBenjamin A Garcia
Aug 21, 2007·Expert Review of Proteomics·Morten Beck Trelle, Ole Nørregaard Jensen
Aug 21, 2007·Expert Review of Proteomics·Michael S Cosgrove
Apr 2, 2009·Expert Opinion on Investigational Drugs·Peter Gimsing
Feb 8, 2016·Breast Cancer Research : BCR·Marsela BraunsteinMelanie Spears
May 26, 2012·Journal of Proteomics·Rune MatthiesenJesus M Arizmendi
Mar 6, 2012·International Journal of Mass Spectrometry·Elisabeth HersmanRobert J Cotter
Sep 8, 2010·Journal of Neuroscience Methods·Pritika J Narayan, Michael Dragunow
Apr 26, 2011·International Journal of Mass Spectrometry·Lei Xiong, Yinsheng Wang
Mar 23, 2010·Journal of Theoretical Biology·Sonja J ProhaskaDavid C Krakauer
Jun 11, 2008·Journal of Proteomics·Alexandre PanchaudMartin Kussmann
Dec 13, 2006·Current Opinion in Chemical Biology·Benjamin A GarciaDonald F Hunt
Jul 23, 2009·Chembiochem : a European Journal of Chemical Biology·Kyle P ChiangTom W Muir

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