Quantitative proteomic analysis of the effects of a GalNAc/Man-specific lectin CSL on yeast cells by label-free LC-MS

International Journal of Biological Macromolecules
Shuai LiuWei Li

Abstract

A Ca(2+)-dependent GalNAc/Man-specific lectin (CSL) from Cyclina sinensis was isolated, and its stimulatory action was characterized in yeast. CSL showed a potent effect on the production of ethanol by Saccharomyces cerevisiae. In this work, the changes in the protein expression profiles of S. cerevisiae after 24h of incubation with CSL were analyzed using label-free quantitative proteomics. A total of 1410 proteins were identified, but only 117 proteins showed significant differences in normalized volume (p<0.05). Among the latter proteins, 24 proteins were up-regulated, and 93 were down-regulated. Analysis of the proteome revealed that CSL triggered changes in the concentrations of some enzymes, such as increased expression of hexokinase, glyceraldehyde 3-phosphate dehydrogenase and enolase and decreased expression of dihydrolipoamide dehydrogenase and aldehyde dehydrogenase. These results indicate that CSL can cause some changes in the metabolic pathway involved in ethanol synthesis in S. cerevisiae. These data may help us understand the stimulatory mechanism of lectin in the fermentation process.

References

Jul 17, 1998·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·C S Tsai, Q Chen
Jun 7, 2006·Molecular Immunology·Shigeyuki TsutsuiTasuku Watanabe
Feb 12, 2008·International Journal of Food Microbiology·Marina BelyDenis Dubourdieu
Mar 14, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Keisuke G TakahashiKiyokuni Muroga
Apr 22, 2008·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Roger R DreschMagdolna M Vozári-Hampe
Jun 6, 2008·Bioresource Technology·Mirbella Cáceres-FarfánLuis Barahona-Pérez
Nov 29, 2008·Journal of Proteomics·Amber L MosleyMichael P Washburn
Apr 21, 2009·Nature Methods·Jacek R WiśniewskiMatthias Mann
May 14, 2011·Analytica Chimica Acta·Maria E YakovlevaBengt Danielsson
May 20, 2011·Nature·Björn SchwanhäusserMatthias Selbach
Sep 17, 2013·PloS One·Basti BergdahlMarie F Gorwa-Grauslund
Dec 24, 2013·International Journal of Food Microbiology·Jaime Moreno-GarcíaJuan Carlos Mauricio
Jan 18, 2014·Molecular & Cellular Proteomics : MCP·Nadia MatesThomas M Kriegel
Aug 12, 2014·Journal of Proteomics·Keyun WangHanfa Zou

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.