Quantitative proteomic analysis of the rice (Oryza sativa L.) salt response

PloS One
Jianwen XuJi Huang

Abstract

Salt stress is one of most serious limiting factors for crop growth and production. An isobaric Tags for Relative and Absolute Quantitation (iTRAQ) approach was used to analyze proteomic changes in rice shoots under salt stress in this study. A total of 56 proteins were significantly altered and 16 of them were enriched in the pathways of photosynthesis, antioxidant and oxidative phosphorylation. Among these 16 proteins, peroxiredoxin Q and photosystem I subunit D were up-regulated, while thioredoxin M-like, thioredoxin x, thioredoxin peroxidase, glutathione S-transferase F3, PSI subunit H, light-harvesting antenna complex I subunits, chloroplast chaperonin, vacuolar ATP synthase subunit H, and ATP synthase delta chain were down-regulated. Moreover, physiological data including total antioxidant capacity, peroxiredoxin activity, chlorophyll a/b content, glutathione S-transferase activity, reduced glutathione content and ATPase activity were consistent with changes in the levels of these proteins. The levels of the mRNAs encoding these proteins were also analyzed by real-time quantitative reverse transcription PCR, and approximately 86% of the results were consistent with the iTRAQ data. Importantly, our data suggest the importa...Continue Reading

References

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Citations

Jan 27, 2016·Plant Signaling & Behavior·Jianwen XuJi Huang
Jul 26, 2016·Frontiers in Plant Science·Yongxue ZhangBing Yu
Dec 1, 2018·BMC Plant Biology·Jiaowen PanWei Liu
May 26, 2018·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Libao ChengShuyan Li
Aug 26, 2020·International Journal of Molecular Sciences·Qiushuo SongVijay Joshi
Feb 25, 2017·Frontiers in Plant Science·Kamlesh K MeenaParamjit S Minhas

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Methods Mentioned

BETA
electrophoresis
reverse transcription PCR
salt stress
proteomic profiling
AsA

Software Mentioned

MapMan
Mascot

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