Quantitative proteomic characterization of cellular pathways associated with altered insulin sensitivity in skeletal muscle following high-fat diet feeding and exercise training

Scientific Reports
Maximilian KleinertLykke Sylow

Abstract

Regular exercise elicits advantageous metabolic adaptations in skeletal muscle, such as improved insulin sensitivity. However, the underpinning molecular mechanisms and the effect of diet on muscle exercise training benefits are unclear. We therefore characterized the skeletal muscle proteome following exercise training (ET) in mice fed chow or high-fat diet (HFD). ET increased exercise performance, lowered body-weight, decreased fat mass and improved muscle insulin action in chow- and HFD-fed mice. At the molecular level, ET regulated 170 muscle proteins in chow-fed mice, but only 29 proteins in HFD-fed mice. HFD per se altered 56 proteins, most of which were regulated in a similar direction by ET. To identify proteins that might have particular health-related bearing on skeletal muscle metabolism, we filtered for differentially regulated proteins in response to ET and HFD. This yielded 15 proteins, including the major urinary protein 1 (MUP1), which was the protein most decreased after HFD, but increased with ET. The ET-induced Mup1 expression was absent in mouse muscle lacking functional AMPK. MUP1 also potentiated insulin-stimulated GLUT4 translocation in cultured muscle cells. Collectively, we provide a resource of ET-regu...Continue Reading

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Citations

Apr 19, 2019·Medicine and Science in Sports and Exercise·Yongchul JangYoungil Lee
Sep 24, 2020·Nature Reviews. Endocrinology·Andreas Mæchel FritzenBente Kiens
May 29, 2019·Annual Review of Nutrition·Andreas Mæchel FritzenBente Kiens
Jul 14, 2020·Frontiers in Cell and Developmental Biology·Ivo Vieira de Sousa NetoRita de Cassia Marqueti
Mar 28, 2020·Nature Communications·Steffen H RaunLykke Sylow
Jun 3, 2021·Proteomes·Mriga DuttBenjamin L Parker
Jun 27, 2020·Cell·James A SanfordUNKNOWN Molecular Transducers of Physical Activity Consortium
Aug 25, 2020·Journal of Proteome Research·Kathryn R JacobsonSarah Calve
Nov 10, 2021·The Biochemical Journal·Erik A RichterMark Hargreaves
Dec 16, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Carina BoddenAnthony J Hannan
Dec 3, 2021·Molecular Metabolism·Marc Leal-JuliàAssumpció Bosch

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Methods Mentioned

BETA
PCR

Software Mentioned

Perseus
FISHER
- Slim
SequestHT
Proteome Discoverer
GO
PANTHER

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