Quantitative proteomics analysis of macrophage-derived lipid rafts reveals induction of autophagy pathway at the early time of Francisella tularensis LVS infection

Journal of Proteome Research
Anetta HärtlovaJirí Stulík

Abstract

Francisella tularensis is a highly infectious intracellular pathogen that has evolved an efficient strategy to subvert host defense response to survive inside the host. The molecular mechanisms regulating these host-pathogen interactions and especially those that are initiated at the time of the bacterial entry via its attachment to the host plasma membrane likely predetermine the intracellular fate of pathogen. Here, we provide the evidence that infection of macrophages with F. tularensis leads to changes in protein composition of macrophage-derived lipid rafts, isolated as detergent-resistant membranes (DRMs). Using SILAC-based quantitative proteomic approach, we observed the accumulation of autophagic adaptor protein p62 at the early stages of microbe-host cell interaction. We confirmed the colocalization of the p62 with ubiquitinated and LC3-decorated intracellular F. tularensis microbes with its maximum at 1 h postinfection. Furthermore, the infection of p62-knockdown host cells led to the transient increase in the intracellular number of microbes up to 4 h after in vitro infection. Together, these data suggest that the activation of the autophagy pathway in F. tularensis infected macrophages, which impacts the early phase...Continue Reading

References

Jun 5, 1997·Nature·K Simons, E Ikonen
Dec 2, 2000·Science·K Simons, E Ikonen
Dec 5, 2000·Current Biology : CB·C M RosenbergerB B Finlay
Jun 21, 2001·JAMA : the Journal of the American Medical Association·D T DennisUNKNOWN Working Group on Civilian Biodefense
Jan 9, 2003·Journal of Lipid Research·Peter Gargalovic, Ladislav Dory
Mar 1, 2003·The European Respiratory Journal·A Tärnvik, L Berglund
May 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Leonard J FosterMatthias Mann
May 1, 1961·Archives of Internal Medicine·S SASLAWS CARHART
Sep 11, 2004·Journal of Medical Microbiology·Helena LindgrenAnders Sjöstedt
Aug 23, 2005·Infection and Immunity·Daniel L ClemensMarcus A Horwitz
Oct 1, 2005·Analytical Chemistry·Martin GilarJohn C Gebler
Nov 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Catharine M Bosio, Steven W Dow
Sep 20, 2006·Proceedings of the National Academy of Sciences of the United States of America·Claire ChecrounJean Celli
Dec 2, 2006·Nature Reviews. Molecular Cell Biology·Matthias Mann
Dec 19, 2006·Trends in Biochemical Sciences·Jorge MoscatMarie W Wooten
Jun 21, 2007·The Journal of Biological Chemistry·Serhiy PankivTerje Johansen
Jun 5, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Batcha Tamilselvam, Simon Daefler
Aug 15, 2008·Journal of Proteome Research·Wilfred H TangSean L Seymour
Nov 26, 2008·Autophagy·Thomas J CremerJonathan P Butchar
Oct 9, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yiyu T ZhengJohn H Brumell
Dec 17, 2009·Methods in Molecular Biology·Joshua E Elias, Steven P Gygi
Apr 7, 2010·Methods : a Companion to Methods in Enzymology·Vasudev J BaileyTza-Huei Wang
Apr 10, 2010·Microbiology and Immunology·Anetta HartlovaJiri Stulik
Sep 4, 2010·Autophagy·Sarah WatersCaroline A Whitehouse
Dec 30, 2010·Autophagy·Terje Johansen, Trond Lamark

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Citations

Jul 28, 2016·Journal of Molecular Biology·Cheryl Miller, Jean Celli
Jul 25, 2019·Frontiers in Cellular and Infection Microbiology·Klara Kubelkova, Ales Macela

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