Quantitative Proteomics Analysis Reveals Novel Insights into Mechanisms of Action of Long Noncoding RNA Hox Transcript Antisense Intergenic RNA (HOTAIR) in HeLa Cells.

Molecular & Cellular Proteomics : MCP
Peng ZhengFeng Ge

Abstract

Long noncoding RNAs (lncRNAs), which have emerged in recent years as a new and crucial layer of gene regulators, regulate various biological processes such as carcinogenesis and metastasis. HOTAIR (Hox transcript antisense intergenic RNA), a lncRNA overexpressed in most human cancers, has been shown to be an oncogenic lncRNA. Here, we explored the role of HOTAIR in HeLa cells and searched for proteins regulated by HOTAIR. To understand the mechanism of action of HOTAIR from a systems perspective, we employed a quantitative proteomic strategy to systematically identify potential targets of HOTAIR. The expression of 170 proteins was significantly dys-regulated after inhibition of HOTAIR, implying that they could be potential targets of HOTAIR. Analysis of this data at the systems level revealed major changes in proteins involved in diverse cellular components, including the cytoskeleton and the respiratory chain. Further functional studies on vimentin (VIM), a key protein involved in the cytoskeleton, revealed that HOTAIR exerts its effects on migration and invasion of HeLa cells, at least in part, through the regulation of VIM expression. Inhibition of HOTAIR leads to mitochondrial dysfunction and ultrastructural alterations, su...Continue Reading

References

Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M H HeggenessS J Singer
Jun 1, 1989·The Journal of Pathology·W A Raymond, A S Leong
Nov 1, 1995·Journal of Clinical Pathology·M HeatleyP Toner
Jul 29, 1996·International Journal of Cancer. Journal International Du Cancer·R SeshadriK McCaul
Jan 18, 2006·Methods in Enzymology·Katalin F Medzihradszky
May 22, 2007·Experimental Cell Research·Johanna IvaskaJohn E Eriksson
Nov 7, 2007·The Biochemical Journal·Ho Lam TangMing Chiu Fung
Jan 26, 2008·Cell Death and Differentiation·T Klimova, N S Chandel
Mar 1, 2008·Circulation Research·Jeong-A KimJames R Sowers
Dec 2, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Neil R KitteringhamB Kevin Park
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Feb 7, 2009·Nature Nanotechnology·M Lekka, P Laidler
Feb 19, 2009·Cancer Research·Torsten W RemmerbachJochen Guck
Feb 26, 2009·Cell·Chris P PontingWolf Reik
Jun 6, 2009·Expert Review of Proteomics·Kyunggon Kim, Youngsoo Kim
Jul 10, 2010·Science·Miao-Chih TsaiHoward Y Chang
Sep 25, 2010·Cell Cycle·Yue Wan, Howard Y Chang
Mar 24, 2011·Journal of Structural Biology·Marija PlodinecCora-Ann Schoenenberger
Apr 15, 2011·Molecular Cancer·Ewan A GibbWan L Lam
May 13, 2011·Molecular Biology of the Cell·Oxana E NekrasovaAlexander A Minin
May 20, 2011·Nature·Björn SchwanhäusserMatthias Selbach
Jun 4, 2011·Cellular and Molecular Life Sciences : CMLS·Arun Satelli, Shulin Li
Sep 24, 2008·Nanotechnology·Sarah E CrossJames K Gimzewski
Aug 30, 2011·Nature·Mitchell GuttmanEric S Lander
Oct 13, 2011·PloS One·Ewan A GibbWan L Lam
Nov 19, 2011·Nature Reviews. Genetics·Manel Esteller
Jan 20, 2012·Cancer Research·Takeshi NiinumaYasuhisa Shinomura
Feb 1, 2012·The Journal of International Medical Research·Y J GengG Y Wang
Feb 3, 2012·Journal of Cancer Research and Clinical Oncology·Tatsuro WatanabeMasami Suganuma
Feb 4, 2012·Trends in Biochemical Sciences·Jennifer F Kugel, James A Goodrich

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Citations

Aug 9, 2015·Journal of Proteomics·Qian XiongFeng Ge
Dec 31, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Li PengGuan-Cheng Li
May 18, 2016·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Xing-Dong XiongJin-Ming Yang
Feb 15, 2018·Endocrine-related Cancer·Carolyn M Klinge
Apr 2, 2016·Science Advances·Bony De Kumar, Robb Krumlauf
Jan 20, 2020·Journal of Cellular and Molecular Medicine·Lin CongMingyuan Huang
Aug 23, 2019·Genetic Testing and Molecular Biomarkers·Dina M IsmailRasha M Hussein
Sep 9, 2020·Open Biology·Andrea Cerase, Gian Gaetano Tartaglia
Mar 18, 2020·Frontiers in Genetics·Mirjana Gusic, Holger Prokisch
Apr 26, 2020·International Journal of Molecular Sciences·Yang-Hsiang Lin
Feb 1, 2020·Redox Biology·Carolyn M Klinge
Mar 17, 2021·Investigative Ophthalmology & Visual Science·Saumik BiswasSubrata Chakrabarti
Sep 21, 2021·Proteomics. Clinical Applications·Peng ZhengTongcun Zhang

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