Quantitative Proteomics Analysis Reveals the Min System of Escherichia coli Modulates Reversible Protein Association with the Inner Membrane.

Molecular & Cellular Proteomics : MCP
Hsiao-Lin LeeYu-Ling Shih

Abstract

The Min system of Escherichia coli mediates placement of the division septum at the midcell. It oscillates from pole to pole to establish a concentration gradient of the division inhibition that is high at the poles but low at the midcell; the cell middle thereby becomes the most favorable site for division. Although Min oscillation is well studied from molecular and biophysical perspectives, it is still an enigma as to whether such a continuous, energy-consuming, and organized movement of the Min proteins would affect cellular processes other than the division site selection. To tackle this question, we compared the inner membrane proteome of the wild-type and Δmin strains using a quantitative approach. Forty proteins that showed differential abundance on the inner membrane of the mutant cells were identified and defined as proteins of interest (POIs). More than half of the POIs were peripheral membrane proteins, suggesting that the Min system affects mainly reversible protein association with the inner membrane. In addition, 6 out of 10 selected POIs directly interacted with at least one of the Min proteins, confirming the correlation between POIs and the Min system.Further analysis revealed a functional relationship between ...Continue Reading

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Citations

Nov 26, 2016·ELife·Yaron Caspi, Cees Dekker
Jul 20, 2019·The Plant Journal : for Cell and Molecular Biology·Yi-Ting LiaoHao-Ching Wang
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Jul 19, 2019·Cellular and Molecular Life Sciences : CMLS·Beatrice RammPetra Schwille

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Datasets Mentioned

BETA
PXD002548

Methods Mentioned

BETA
two-hybrid
pull-down
PCR
interaction predictions
B2H
pull down

Software Mentioned

Proteome Discoverer
Dynamic Localizome
mentha
PSORT
PSORTb
IntAct
ASKA clone
PSORTdb
Bruker Daltonics
ImageJ

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