Quantitative proteomics identifies the core proteome of exosomes with syntenin-1 as the highest abundant protein and a putative universal biomarker.

Nature Cell Biology
Fernanda G KugeratskiRaghu Kalluri

Abstract

Exosomes are extracellular vesicles derived from the endosomal compartment that are potentially involved in intercellular communication. Here, we found that frequently used biomarkers of exosomes are heterogeneous, and do not exhibit universal utility across different cell types. To uncover ubiquitous and abundant proteins, we used an unbiased and quantitative proteomic approach based on super-stable isotope labeling with amino acids in cell culture (super-SILAC), coupled to high-resolution mass spectrometry. In total, 1,212 proteins were quantified in the proteome of exosomes, irrespective of the cellular source or isolation method. A cohort of 22 proteins was universally enriched. Fifteen proteins were consistently depleted in the proteome of exosomes compared to cells. Among the enriched proteins, we identified biogenesis-related proteins, GTPases and membrane proteins, such as CD47 and ITGB1. The cohort of depleted proteins in exosomes was predominantly composed of nuclear proteins. We identified syntenin-1 as a consistently abundant protein in exosomes from different cellular origins. Syntenin-1 is also present in exosomes across different species and biofluids, highlighting its potential use as a putative universal biomar...Continue Reading

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Citations

Jul 21, 2021·Life Science Alliance·Kathleen M McAndrewsRaghu Kalluri
Oct 19, 2021·Frontiers in Cell and Developmental Biology·Hyobin Julianne LimDae-Kyum Kim
Nov 9, 2021·Mass Spectrometry Reviews·Iqbal JalaludinJeongkwon Kim
Nov 25, 2021·Journal of Extracellular Vesicles·Alin RaiDavid W Greening
Nov 30, 2021·Journal of Cell Communication and Signaling·Sukhbir KaurDavid D Roberts

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Software Mentioned

GraphPad Prism
Compass
Cytoscape
ExoCarta
MaxQuant
STRING
Primer
FlowJo
SILAC
Xcalibur

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