Apr 18, 2009

Quantitative proteomics investigation of pancreatic intraepithelial neoplasia

Electrophoresis
Sheng PanTeresa A Brentnall

Abstract

Patients with pancreatic cancer are usually diagnosed at late stages, when the disease is incurable. Pancreatic intraepithelial neoplasia (PanIN) 3 is believed to be the immediate precursor lesion of pancreatic adenocarcinoma, and would be an ideal stage to diagnose patients, when intervention and cure are possible and patients are curable. In this study, we used quantitative proteomics to identify dysregulated proteins in PanIN 3 lesions. Altogether, over 200 dysregulated proteins were identified in the PanIN 3 tissues, with a minimum of a 1.75-fold change compared with the proteins in normal pancreas. These dysregulated PanIN 3 proteins play roles in cell motility, the inflammatory response, the blood clotting cascade, the cell cycle and its regulation, and protein degradation. Further network analysis of the proteins identified c-MYC as an important regulatory protein in PanIN 3 lesions. Finally, three of the overexpressed proteins, laminin beta-1, galectin-1, and actinin-4 were validated by immunohistochemistry analysis. All three of these proteins were overexpressed in the stroma or ductal epithelial cells of advanced PanIN lesions as well as in pancreatic cancer tissue. Our findings suggest that these three proteins may b...Continue Reading

Mentioned in this Paper

Gene Expression Regulation, Neoplastic
Protein Degradation, Regulatory
Immunohistochemistry
Systemic Inflammatory Response Syndrome
Cell Motility
Stroma
Pancreatic Carcinoma
Squamous Transitional Epithelial Cell Count
Pancreatitis
ACTN4 gene

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