Quantitative proteomics of Lactococcus lactis F44 under cross-stress of low pH and lactate

Journal of Dairy Science
Hao WuJianjun Qiao

Abstract

Lactococcus lactis encounters 3 environmental stimuli, H+, lactate, and undissociated lactic acid, because of the accumulation of lactic acid-the predominant fermentation product. Few studies have examined how these stimuli synergistically affect the bacteria. Herein, we analyzed the dissociation degree of lactic acid at different pH and investigated the cellular response to cross-stress in L. lactis ssp. lactis F44 through quantitative proteomic analysis using isobaric tags for relative and absolute quantitation of 3 groups: 0% lactic acid with pH 4.0 and 0% lactic acid with pH 5.0 for acid stress; 2% lactic acid with pH 7.0 and 3% lactic acid with pH 7.0 for lactate stress; and 2% lactic acid with pH 4.0, 2% lactic acid with pH 5.0, 3% lactic acid with pH 4.0, and 3% lactic acid with pH 5.0 for cross-stress. We observed that the metabolisms of carbohydrate and energy were inhibited, which might be due to the feedback inhibition of lactic acid. The arginine deiminase pathway was improved to maintain the stability of intracellular pH. Additionally, some differentially expressed genes associated with the general stress response, amino acid metabolism, cell wall synthesis, and regulatory systems played significant roles in stress...Continue Reading

References

Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R A VanBogelen, F C Neidhardt
Jan 1, 1987·Applied and Environmental Microbiology·R E MarquisA Wong
Nov 5, 1997·Advances in Microbial Physiology·J B Russell, F Diez-Gonzalez
Jul 2, 1999·Applied and Environmental Microbiology·H ShimizuS Shioya
Nov 21, 2002·Journal of Basic Microbiology·Kathryn A Durham, George S Bullerjahn
Sep 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Paul D Cotter, Colin Hill
Oct 7, 2003·International Journal of Food Microbiology·Dorte FreesHanne Ingmer
May 11, 2004·Microbiology·Alice C L LenNicholas A Jacques
Jun 24, 2004·The Journal of Biological Chemistry·Rachael M Morgan-Kiss, John E Cronan
Oct 21, 2005·Proteomics·Aurélie Budin-VerneuilEmmanuelle Maguin
Jul 18, 2006·Metabolic Engineering·Wietske A PoolOscar P Kuipers
Aug 9, 2006·Critical Reviews in Microbiology·María Fernández, Manuel Zúñiga
Sep 26, 2006·Applied and Environmental Microbiology·S PenaudM van de Guchte
Jul 3, 2007·Applied and Environmental Microbiology·Juan ZhangJian Chen
Jul 24, 2007·Applied and Environmental Microbiology·Yulia RedkoMarie-Pierre Chapot-Chartier
Aug 28, 2007·Applied and Environmental Microbiology·Borja SánchezMonique Zagorec
Jul 5, 1995·Biotechnology and Bioengineering·D D Axe, J E Bailey
Aug 1, 2009·The International Journal of Biochemistry & Cell Biology·Kazuei Igarashi, Keiko Kashiwagi
Mar 9, 2010·Journal of Bacteriology·Jeff R BroadbentJames L Steele
Apr 14, 2010·Molecules and Cells·Kyung-hee KangIngnyol Jin
Mar 23, 2011·Applied and Environmental Microbiology·Thi Mai Huong ToRaphaëlle Tourdot-Maréchal
Jun 28, 2011·Proceedings of the National Academy of Sciences of the United States of America·Bjarne JochimsenBjarne Hove-Jensen
Nov 29, 2011·Journal of Proteomics·Johanna KoponenPekka Varmanen
Feb 19, 2013·International Journal of Food Microbiology·Asa RosengrenRoland Lindqvist
Mar 30, 2013·Biotechnology Letters·Xiao ZhangHonglei Liu
Sep 13, 2013·Nature Reviews. Molecular Cell Biology·Helen Saibil

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Citations

Aug 16, 2018·Scientific Reports·Zeeshan HamidAndrea Armirotti

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