Quantitative proteomics of nuclear phosphoproteins in the root tip of soybean during the initial stages of flooding stress

Journal of Proteomics
Xiaojian Yin, Setsuko Komatsu

Abstract

Soybean is sensitive to flooding stress, which has affected many proteins in roots. To identify the upstream events controlling the regulation of flooding-responsive proteins, nuclear phosphoproteomics of soybean-root tip was performed. Nuclei were isolated from the root tip of 2-day-old soybeans treated with flooding for 3h. The purity of nuclear fractions was confirmed by Western blotting and enzyme-activity assays for subcellular-specific enzymes. Phosphopeptides in the fractions were enriched and analyzed using gel-free proteomic technique. Fourteen phosphoproteins significantly changed in root tip in response to flooding stress. Of these phosphoproteins, 10 proteins including 5 protein synthesis-related proteins were predicted to be localized in the nucleus. In particular, zinc finger/BTB domain-containing protein 47, glycine-rich protein, and rRNA processing protein Rrp5, which are related to abscisic acid (ABA) response, were clearly phosphorylated in response to flooding stress. The mRNA expression levels of these nuclear phosphoproteins were down-regulated in root tip exposed to flooding stress with ABA. In addition, the fresh weight of soybean decreased under flooding stress with ABA, although the fresh weight of plan...Continue Reading

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Citations

Jan 26, 2016·Journal of Proteomics·Xin Wang, Setsuko Komatsu
Mar 24, 2016·New Biotechnology·Xiaojian Yin, Setsuko Komatsu
Aug 4, 2015·Journal of Proteome Research·Setsuko KomatsuYohei Nanjo
Apr 27, 2017·Plant & Cell Physiology·Wen-Jia PanJin-Song Zhang
Jun 20, 2017·The Plant Journal : for Cell and Molecular Biology·Titouan BonnotPierre Martre
Feb 24, 2018·Frontiers in Plant Science·Klára KosováJenny Renaut
Mar 2, 2017·Proteomes·Akiko Hashiguchi, Setsuko Komatsu
Feb 16, 2021·Frontiers in Genetics·Mu-Chien LaiChung-Feng Kao
Feb 3, 2021·International Journal of Molecular Sciences·Muhammad Khuram RazzaqKadambot H M Siddique
Jan 23, 2020·Journal of Proteome Research·Nagib AhsanJán A Miernyk
Oct 16, 2020·International Journal of Molecular Sciences·Xin Wang, Setsuko Komatsu
Jul 13, 2021·Frontiers in Plant Science·Qichao WangKai Shu

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