Quantitative proteomics reveal distinct protein regulations caused by Aggregatibacter actinomycetemcomitans within subgingival biofilms

PloS One
Kai BaoGeorgios N Belibasakis

Abstract

Periodontitis is an infectious disease that causes the inflammatory destruction of the tooth-supporting (periodontal) tissues, caused by polymicrobial biofilm communities growing on the tooth surface. Aggressive periodontitis is strongly associated with the presence of Aggregatibacter actinomycetemcomitans in the subgingival biofilms. Nevertheless, whether and how A. actinomycetemcomitans orchestrates molecular changes within the biofilm is unclear. The aim of this work was to decipher the interactions between A. actinomycetemcomitans and other bacterial species in a multi-species biofilm using proteomic analysis. An in vitro 10-species "subgingival" biofilm model, or its derivative that included additionally A. actinomycetemcomitans, were anaerobically cultivated on hydroxyapatite discs for 64 h. When present, A. actinomycetemcomitans formed dense intra-species clumps within the biofilm mass, and did not affect the numbers of the other species in the biofilm. Liquid chromatography-tandem mass spectrometry was used to identify the proteomic content of the biofilm lysate. A total of 3225 and 3352 proteins were identified in the biofilm, in presence or absence of A. actinomycetemcomitans, respectively. Label-free quantitative pro...Continue Reading

References

Jan 1, 1984·Annual Review of Biochemistry·M NomuraG Baughman
Sep 24, 1998·Caries Research·D J Bradshaw, P D Marsh
Mar 28, 2001·Journal of Dental Research·B GuggenheimS Shapiro
Mar 26, 2004·Nature Reviews. Microbiology·Luanne Hall-StoodleyPaul Stoodley
Dec 23, 2004·Oral Microbiology and Immunology·A SharmaH K Kuramitsu
Jan 18, 2005·FEMS Microbiology Reviews·Teresa OlczakCaroline Attardo Genco
Apr 28, 2005·Periodontology 2000·Sigmund S Socransky, Anne D Haffajee
Nov 8, 2005·Journal of Clinical Microbiology·Jørn A AasFloyd E Dewhirst
May 2, 2006·Statistical Applications in Genetics and Molecular Biology·Gordon K Smyth
Jun 27, 2006·Molecular Microbiology·Alexander H RickardPaul E Kolenbrander
Aug 26, 2006·Periodontology 2000·Adam P Roberts, Peter Mullany
Aug 26, 2006·Periodontology 2000·Paul E KolenbranderPatricia I Diaz
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·Josef DeutscherPieter W Postma
Jan 9, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Eric R RhodesLuis A Actis
Jan 11, 2007·Infection and Immunity·Lakshmyya KesavaluJeffrey L Ebersole
Jun 26, 2007·Journal of Bacteriology·Stacie A Brown, Marvin Whiteley
Dec 8, 2007·Microbiology and Molecular Biology Reviews : MMBR·Howard K KuramitsuWenyuan Shi
Apr 21, 2009·Nature Methods·Jacek R WiśniewskiMatthias Mann
Mar 3, 2010·PloS One·Vincent ZijngeHermie J M Harmsen
Mar 5, 2010·Journal of Dental Research·S C Kachlany
Jun 2, 2010·Nature Reviews. Microbiology·Richard P Darveau
Aug 18, 2010·Periodontology 2000·Brian HendersonDerren Ready
Apr 13, 2011·PLoS Pathogens·Matthew M RamseyMarvin Whiteley
May 10, 2011·Cellular Immunology·Nagihan BostanciGeorgios N Belibasakis
Jul 27, 2011·PloS One·Fran SupekTomislav Šmuc
Apr 26, 2012·FEMS Microbiology Letters·Nagihan Bostanci, Georgios N Belibasakis
Jul 10, 2012·Journal of Dental Research·R P DarveauM A Curtis
Jul 20, 2012·Journal of Proteome Research·Zamirah Zainal-AbidinEric C Reynolds
Sep 4, 2012·Nature Reviews. Microbiology·George HajishengallisMichael A Curtis
Sep 20, 2012·BMC Microbiology·Erik L HendricksonMurray Hackett

❮ Previous
Next ❯

Citations

Mar 22, 2016·Anaerobe·Jôice Dias CorrêaTarcília Aparecida da Silva
Oct 16, 2015·Clinical Oral Implants Research·Thomas Thurnheer, Georgios N Belibasakis
Jul 25, 2015·Current Opinion in Microbiology·Olga Ponomarova, Kiran Raosaheb Patil
Dec 21, 2016·Proteomics·Nagihan Bostanci, Kai Bao
Jul 30, 2019·Journal of Dental Research·D P MillerR J Lamont
Nov 9, 2019·Pathogens·Georgios N BelibasakisAnders Johansson
Dec 14, 2018·Disease Markers·Alejandro I Lorenzo-PousoAbel García-García
Jul 2, 2017·Scientific Reports·Kai BaoGeorgios N Belibasakis
May 11, 2018·Clinical and Experimental Dental Research·Gabriela Dabija-WolterAnne Isine Bolstad
May 10, 2019·Frontiers in Cellular and Infection Microbiology·Maribasappa KarchedSirkka Asikainen
Aug 17, 2019·Proteomics. Clinical Applications·Nurul Iman Binti Badlishah ShamMelissa M Grant
Apr 29, 2016·PloS One·Shalini Singh, Vikash Kumar Dubey
Oct 9, 2020·Critical Reviews in Microbiology·Chaminda J SeneviratneGordon Ramage
Nov 24, 2020·Periodontology 2000·Nagihan BostanciGeorgios N Belibasakis
Nov 24, 2020·Periodontology 2000·Bhumika ShokeenRenate Lux
Mar 3, 2021·Critical Reviews in Microbiology·Faizan Ahmed SadiqHao Zhang
May 30, 2021·Nature Reviews. Microbiology·Adrian TettNicola Segata

❮ Previous
Next ❯

Methods Mentioned

BETA
protein folding
PCR
Protein Assay

Software Mentioned

Scaffold
REVIGO
Reduce + Visualize Gene Ontology ( REVIGO )
Prism
SafeQuant
Primer
Mascot
- BLAST
Progenesis LC — MS
Imaris

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

Bacterial Protein Structures

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.