Quantitative proteomics reveals protein profiles underlying major transitions in aspen wood development

BMC Genomics
Ogonna ObuduluGunnar Wingsle

Abstract

Wood development is of outstanding interest both to basic research and industry due to the associated cellulose and lignin biomass production. Efforts to elucidate wood formation (which is essential for numerous aspects of both pure and applied plant science) have been made using transcriptomic analyses and/or low-resolution sampling. However, transcriptomic data do not correlate perfectly with levels of expressed proteins due to effects of post-translational modifications and variations in turnover rates. In addition, high-resolution analysis is needed to characterize key transitions. In order to identify protein profiles across the developmental region of wood formation, an in-depth and tissue specific sampling was performed. We examined protein profiles, using an ultra-performance liquid chromatography/quadrupole time of flight mass spectrometry system, in high-resolution tangential sections spanning all wood development zones in Populus tremula from undifferentiated cambium to mature phloem and xylem, including cell expansion and cell death zones. In total, we analyzed 482 sections, 20-160 μm thick, from four 47-year-old trees growing wild in Sweden. We obtained high quality expression profiles for 3,082 proteins exhibiting...Continue Reading

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Citations

Oct 9, 2016·Plant Science : an International Journal of Experimental Plant Biology·Christoffer Johnsson, Urs Fischer
Feb 23, 2019·Journal of Experimental Botany·Rafael Andrade BuonoMoritz K Nowack
Jun 22, 2017·Journal of Experimental Botany·Joakim BygdellGunnar Wingsle
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Methods Mentioned

BETA
proteomic profiling
Protein Assay
acetylation
PCA

Software Mentioned

MAPMAN
OPLS
PermutMatrix
SIMCA
DA
Protein Lynx Global Server

Related Concepts