Quantitative Proteomics Uncovers Novel Factors Involved in Developmental Differentiation of Trypanosoma brucei

PLoS Pathogens
Mario DejungChristian J Janzen

Abstract

Developmental differentiation is a universal biological process that allows cells to adapt to different environments to perform specific functions. African trypanosomes progress through a tightly regulated life cycle in order to survive in different host environments when they shuttle between an insect vector and a vertebrate host. Transcriptomics has been useful to gain insight into RNA changes during stage transitions; however, RNA levels are only a moderate proxy for protein abundance in trypanosomes. We quantified 4270 protein groups during stage differentiation from the mammalian-infective to the insect form and provide classification for their expression profiles during development. Our label-free quantitative proteomics study revealed previously unknown components of the differentiation machinery that are involved in essential biological processes such as signaling, posttranslational protein modifications, trafficking and nuclear transport. Furthermore, guided by our proteomic survey, we identified the cause of the previously observed differentiation impairment in the histone methyltransferase DOT1B knock-out strain as it is required for accurate karyokinesis in the first cell division during differentiation. This epigen...Continue Reading

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Oct 26, 2016·Scientific Reports·Zdenka CicovaChristian J Janzen
Mar 14, 2017·Nature Microbiology·Filipa Rijo-FerreiraLuisa M Figueiredo
Apr 11, 2017·PLoS Pathogens·Henriette ZimmermannMarkus Engstler
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Datasets Mentioned

BETA
PXD003319

Methods Mentioned

BETA
dissection
GTPases
GTPase
flow cytometry
PCR
transfection

Software Mentioned

tritrypDB
FlowJo
Andromeda
MaxQuant
ggplot2 R - package
GraphPad Prism
stats R - package
CellQuest
NMF R - package
psych R - package

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African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.