Quantitative, radial diffusion slide assay for staphylocoagulase.

Applied and Environmental Microbiology
J D Kohl, M G Johnson

Abstract

A simple, quantitative radial diffusion assay for staphylocoagulase in culture fluids, using microscope slides coated with a thin layer of agar containing plasma and fibrinogen, was developed. No prior purification of the enzyme was needed, and only small quantities, 7 microliter, were required for each test. This method is particularly suitable for objectively comparing the relative amounts of coagulase produced by different cultures of Staphylococcus aureus.

References

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Nov 1, 1967·Applied Microbiology·C B DonnellyK H Lewis

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