Quantitative real-time PCR does not show selective targeting of p14(ARF) but concomitant inactivation of both p16(INK4A) and p14(ARF) in 105 human primary gliomas

Oncogene
M LabuhnA Merlo

Abstract

In many human cancers, the INK4A locus is frequently mutated by homozygous deletions. By alternative splicing this locus encodes two non-related tumor suppressor genes, p16(INK4A) and p14(ARF) (p19(ARF) in mice), which regulate cell cycle and cell survival in the retinoblastoma protein (pRb) and p53 pathways, respectively. In mice, the role of p16(INK4A) as the critical tumor suppressor gene at the INK4A locus was challenged when it was found that p19(ARF) only knock-out mice developed tumors, including gliomas. We have analysed the genetic status of the INK4A locus in 105 primary gliomas using both microsatellite mapping (MSM) and quantitative real-time PCR (QRT-PCR). Comparison of the results of the two methods revealed agreement in 67% of the tumors examined. In discordant cases, fluorescence in situ hybridization (FISH) analysis was always found to support QRT-PCR classification. Direct assessment of p14(ARF) exon 1beta, p16(INK4A) exon 1alpha and exon 2 by QRT-PCR revealed 43 (41%) homozygous and eight (7%) hemizygous deletions at the INK4A locus. In 49 (47%) gliomas, both alleles were retained. In addition, QRT-PCR, but not MSM, detected hyperploidy in five (5%) tumors. Deletion of p14(ARF) was always associated with co-d...Continue Reading

References

Oct 1, 1995·Nature Genetics·P CairnsD Sidransky
Jul 1, 1993·Brain Pathology·P KleihuesB W Scheithauer
Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·D E QuelleC J Sherr
Oct 6, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E J SpeelA H Hopman
Oct 28, 1998·Genes, Chromosomes & Cancer·R KumarK Hemminki
Dec 18, 1998·Science·P SunD Beach
Feb 6, 1999·International Journal of Cancer. Journal International Du Cancer·B GörtzP Komminoth
Mar 9, 2000·Nature Cell Biology·A CarneroD H Beach
Mar 9, 2000·Nature Cell Biology·A C Lloyd
Sep 20, 2000·Genes & Development·J D WeberG P Zambetti

❮ Previous
Next ❯

Citations

Apr 17, 2008·Brain Tumor Pathology·Yoshiyuki TanakaToru Itakura
Sep 29, 2006·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Cristóbal Belda-IniestaManuel González Barón
Feb 2, 2010·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Elodie LaharanneJean-Philippe Merlio
Apr 13, 2005·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Hiroko Ohgaki
Jul 16, 2002·Gut·A TannapfelC Wittekind
Aug 15, 2008·Cancer Research·Dorothea DouglasElizabeth R Lawlor
Aug 27, 2005·Blood·Kai FuUNKNOWN Lymphoma/Leukemia Molecular Profiling Project
Mar 3, 2009·BMC Cancer·Jean-Louis BoulayFrank Hirth
Oct 14, 2009·PloS One·Serdar KorurMaria Maddalena Lino
Jan 1, 2010·Pharmacogenomics and Personalized Medicine·Jing LiD Cory Adamson
Jun 15, 2014·World Journal of Surgical Oncology·Weiqing WanYouyong Lu
Jun 27, 2009·Expert Opinion on Investigational Drugs·Cory AdamsonDarell D Bigner
Feb 6, 2007·British Journal of Haematology·Andrew J DaviesT Andrew Lister
Jan 15, 2013·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Suvendu PurkaitChitra Sarkar
Feb 24, 2015·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Matthew N T ThuyKatharine J Drummond
Jun 4, 2015·PloS One·Yayoi YoshimuraKenji Tanigaki
Feb 15, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Arnab ChakravartiJay S Loeffler
Mar 4, 2008·Virchows Archiv : an International Journal of Pathology·Marc-Antoine Belaud-RotureauJean-Philippe Merlio
Nov 13, 2001·Oncogene·A TannapfelC Wittekind
Jun 28, 2005·Journal of Neuropathology and Experimental Neurology·Hiroko Ohgaki, Paul Kleihues
Sep 25, 2009·Clinical Medicine. Oncology·Okezie O KanuCory Adamson
May 5, 2009·Expert Opinion on Therapeutic Targets·Okezie O KanuDavid Cory Adamson
Apr 15, 2006·Journal of Neuro-oncology·Muhamad Thohar ArifinKaoru Kurisu
Aug 7, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jaren W LandenDavid R Archer
Mar 12, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Takashi UekiAsif Rashid
Jun 21, 2008·Molecular Cancer Therapeutics·Eisaku KondoTadashi Yoshino
Oct 7, 2004·Cancer Research·Hiroko OhgakiPaul Kleihues

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.