Quantitative RNA-seq Analysis Unveils Osmotic and Thermal Adaptation Mechanisms Relevant for Ectoine Production in Chromohalobacter salexigens

Frontiers in Microbiology
Manuel SalvadorCarmen Vargas

Abstract

Quantitative RNA sequencing (RNA-seq) and the complementary phenotypic assays were implemented to investigate the transcriptional responses of Chromohalobacter salexigens to osmotic and heat stress. These conditions trigger the synthesis of ectoine and hydroxyectoine, two compatible solutes of biotechnological interest. Our findings revealed that both stresses make a significant impact on C. salexigens global physiology. Apart from compatible solute metabolism, the most relevant adaptation mechanisms were related to "oxidative- and protein-folding- stress responses," "modulation of respiratory chain and related components," and "ion homeostasis." A general salt-dependent induction of genes related to the metabolism of ectoines, as well as repression of ectoine degradation genes by temperature, was observed. Different oxidative stress response mechanisms, secondary or primary, were induced at low and high salinity, respectively, and repressed by temperature. A higher sensitivity to H2O2 was observed at high salinity, regardless of temperature. Low salinity induced genes involved in "protein-folding-stress response," suggesting disturbance of protein homeostasis. Transcriptional shift of genes encoding three types of respiratory ...Continue Reading

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Datasets Mentioned

BETA
GSE69612

Methods Mentioned

BETA
PCR
electrophoresis
RNA-seq
Reverse
Assay
thermal stress
protein folding

Software Mentioned

Bioconductor
DAVID
EDASeq
ExprEssence
Cytoscape
SETS ( Solid Experimental Tracking System )
SOLiD
BLAST
DESeq
BioScope

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