Abstract
Recently, an increasing interest has been observed in ionic liquids (ILs) due to their potentialities in various chemical processes. ILs have some unique properties making them excellent additives in CE. In this work a simple, rapid, and reliable CZE method has been developed and validated using 1-butyl-3-methyl imidazolium hexafluorophosphate (BMIM-PF6 ) ionic liquid as a buffer additive for the determination/separation of five flavonoids including hesperedin, epicatechin (EC), epigallocatechin gallate (EGCG), and morin using photodiode array (PDA) detector. The effect of several parameters such as concentration and pH of the running buffer, applied voltage, and concentration of ionic liquid were optimized. CZE at 25°C with 25 mM borate buffer of pH 9.0 at an applied voltage of 17 kV by adding 17.5 mM of IL was found to be suitable for the separation/determination of all five analytes within 08 min. Validation of the method was performed in terms of linearity, accuracy, precision, and limit of detection and quantification. The calibration curves were plotted in the concentration range of 1-200 μg/mL for all five analytes. The response was linear with R2 = 0.990 for EC, chrysin, and hesperidin, 0.992 for morin, and 0.988 for E...Continue Reading
References
Jan 23, 1999·Journal of Chromatography. a·L ArceM Valcárcel
Feb 26, 2000·Journal of Agricultural and Food Chemistry·L Z LinG A Cordell
May 24, 2000·Journal of Chromatography. a·J P AucampZ Apostolides
Jul 25, 2000·Journal of Chromatography. a·B L Lee, C N Ong
Sep 6, 2001·Analytical Chemistry·E G YanesA M Stalcup
Dec 25, 2002·Journal of Chromatography. a·M VaherM Kaljurand
Jan 29, 2003·The Journal of Nutritional Biochemistry·Kelly E. HeimDennis J. Bobilya
Feb 13, 2003·Journal of Chromatography. a·Weidong QinSam Fong Yau Li
Feb 15, 2003·Journal of Chromatography. a·Fengxia Deng, S William Zito
Feb 20, 2003·Journal of Agricultural and Food Chemistry·Matteo BonoliGiovanni Lercker
Jul 31, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Kazuo IshiiYasuji Kasuya
Apr 7, 2004·Journal of Chromatography. a·Shu-Ping Wang, Kuo-Jun Huang
Jul 30, 2004·Journal of Chromatography. a·Yiannis C FiamegosConstantine D Stalikas
Feb 1, 2005·Plant Foods for Human Nutrition·L H YaoS S Chen
Dec 13, 2005·Journal of Chromatography. a·J J LiuY T Wang
Dec 21, 2005·Journal of Pharmaceutical and Biomedical Analysis·Michał Piotr MarszałłRoman Kaliszan
Mar 10, 2006·Journal of Separation Science·Mei-E Yue, Yan-Ping Shi
May 22, 2007·Journal of Pharmaceutical and Biomedical Analysis·Mina LuoShengxiang Jiang
Jun 26, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Gong-Jun YangZhi-Yue Wang
Oct 26, 2007·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Giuseppe GattusoCorrado Caristi
Apr 30, 2007·Talanta·Kan TianZhide Hu
Jul 15, 2011·Food Chemistry·Renato G PeresDelia B Rodriguez-Amaya
Nov 20, 2012·Electrophoresis·Libo LiTianyan You
Apr 20, 2013·Talanta·Yuan-Yuan LiHon-Yeung Cheung
Nov 6, 2013·Journal of Chromatography. a·Yuanqi LuMichael C Breadmore
Dec 24, 2013·Food Chemistry·Chao-Ming LiuYang-Wei Lin
Jun 10, 2014·Journal of Separation Science·Bingbing SunMinglin Wang
Aug 22, 2014·Electrophoresis·Yuanqi LuMichael C Breadmore
Sep 10, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Deia Abd El-Hady, Hassan M Albishri
Aug 16, 2016·Journal of Chromatography. a·Qi ZhangMengjie Rui
Oct 24, 2017·Journal of Pharmaceutical and Biomedical Analysis·Joachim Wahl, Ulrike Holzgrabe