Quantitative SHG imaging in osteoarthritis model mice, implying a diagnostic application

Biomedical Optics Express
Hiroshi KiyomatsuTakeshi Imamura

Abstract

Osteoarthritis (OA) restricts the daily activities of patients and significantly decreases their quality of life. The development of non-invasive quantitative methods for properly diagnosing and evaluating the process of degeneration of articular cartilage due to OA is essential. Second harmonic generation (SHG) imaging enables the observation of collagen fibrils in live tissues or organs without staining. In the present study, we employed SHG imaging of the articular cartilage in OA model mice ex vivo. Consequently, three-dimensional SHG imaging with successive image processing and statistical analyses allowed us to successfully characterize histopathological changes in the articular cartilage consistently confirmed on histological analyses. The quantitative SHG imaging technique presented in this study constitutes a diagnostic application of this technology in the setting of OA.

References

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Citations

Jul 3, 2015·Biomedical Optics Express·Rajesh KumarMagnus B Lilledahl
Jun 18, 2016·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Carina L BlakerChristopher B Little
Oct 12, 2019·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·M Rocio Servin-VencesAnca Margineanu
Mar 16, 2018·Laboratory Investigation; a Journal of Technical Methods and Pathology·Carrie K Hui MingaloneLi Zeng
Apr 5, 2017·Bio-medical Materials and Engineering·Christel HenrionnetAstrid Pinzano
May 24, 2019·Osteoarthritis and Cartilage·S SantosD M Pierce
May 20, 2021·Connective Tissue Research·Ziad AbusaraWalter Herzog

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