Quantitative signature for architectural organization of regulatory factors using intranuclear informatics

Journal of Cell Science
Daniel YoungG S Stein

Abstract

Regulatory machinery for replication and gene expression is punctately organized in supramolecular complexes that are compartmentalized in nuclear microenvironments. Quantitative approaches are required to understand the assembly of regulatory machinery within the context of nuclear architecture and to provide a mechanistic link with biological control. We have developed 'intranuclear informatics' to quantify functionally relevant parameters of spatially organized nuclear domains. Using this informatics strategy we have characterized post-mitotic reestablishment of focal subnuclear organization of Runx (AML/Cbfa) transcription factors in progeny cells. By analyzing point mutations that abrogate fidelity of Runx intranuclear targeting, we establish molecular determinants for the spatial order of Runx domains. Our novel approach provides evidence that architectural organization of Runx factors may be fundamental to their tissue-specific regulatory function.

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Oct 3, 2002·Journal of Cell Science·Kimberly S HarringtonGary S Stein
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Nov 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Karina BarseguianGary S Stein
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Citations

May 25, 2007·Nature Reviews. Cancer·Sayyed K ZaidiGary S Stein
Aug 12, 2005·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Gareth N Corry, D Alan Underhill
Feb 24, 2009·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Gary S SteinMohammad Q Hassan
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Daniel W YoungGary S Stein
Mar 27, 2015·Journal of Cellular Physiology·Robert E ScottGary S Stein
Nov 17, 2009·Comptes rendus biologies·Valérie GaudinPascale Debey
Sep 28, 2005·Birth Defects Research. Part C, Embryo Today : Reviews·Tania M SchroederJennifer J Westendorf
Jul 11, 2008·Journal of Cellular Biochemistry·Gary S SteinMohammad Q Hassan
Nov 7, 2009·Diagnostic Cytopathology·Pranab Dey
Mar 29, 2005·The American Journal of Pathology·Gary S Stein
Dec 25, 2010·Integrative Biology : Quantitative Biosciences From Nano to Macro·Gary S SteinDaniel W Young

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