Quantitative studies of mRNA recruitment to the eukaryotic ribosome

Biochimie
Christopher S Fraser

Abstract

The process of peptide bond synthesis by ribosomes is conserved between species, but the initiation step differs greatly between the three kingdoms of life. This is illustrated by the evolution of roughly an order of magnitude more initiation factor mass found in humans compared with bacteria. Eukaryotic initiation of translation is comprised of a number of sub-steps: (i) recruitment of an mRNA and initiator methionyl-tRNA to the 40S ribosomal subunit; (ii) migration of the 40S subunit along the 5' UTR to locate the initiation codon; and (iii) recruitment of the 60S subunit to form the 80S initiation complex. Although the mechanism and regulation of initiation has been studied for decades, many aspects of the pathway remain unclear. In this review, I will focus discussion on what is known about the mechanism of mRNA selection and its recruitment to the 40S subunit. I will summarize how the 43S preinitiation complex (PIC) is formed and stabilized by interactions between its components. I will discuss what is known about the mechanism of mRNA selection by the eukaryotic initiation factor 4F (eIF4F) complex and how the selected mRNA is recruited to the 43S PIC. The regulation of this process by secondary structure located in the 5...Continue Reading

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Citations

May 25, 2016·Nature Communications·Guo-Liang ChewAlexander F Schier
Sep 24, 2016·Quarterly Reviews of Biophysics·Jin ChenJoseph D Puglisi
Jun 28, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Muhammad Umar AliCao Jiang
Feb 1, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Alex G JohnsonJoseph D Puglisi
Nov 15, 2017·Nucleic Acids Research·Alex G JohnsonJoseph D Puglisi
Apr 7, 2018·Wiley Interdisciplinary Reviews. RNA·Nikolay E Shirokikh, Thomas Preiss
Jul 1, 2018·Cold Spring Harbor Perspectives in Biology·Masaaki Sokabe, Christopher S Fraser
Jun 22, 2019·PLoS Pathogens·William J Neidermyer, Sean P J Whelan
Sep 2, 2015·The Journal of Biological Chemistry·William C Merrick
Nov 26, 2019·Biochemistry·Rishi Kumar MishraTanweer Hussain

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