Quantitative studies of rod--coccus morphogenesis in a temperature-sensitive rod- mutant of Bacillus subtilis

Journal of General Microbiology
I D Burdett


Cells of Bacillus subtilis RodB changed from rods to cocci when shifted from 20 to 42 degrees C in media containing no additional anions. Quantitative studies of surface growth, including cross-wall formation and pole construction, have been made from reconstructions obtained from central, longitudinal sections of cells. Measurements of surface area and volume were obtained by mathematical rotation of axial sections about their longitudinal axis. Surface markers, perhaps analogous to the wall bands of streptococci, have been used to distinguish septal from cylindrical wall. During the shape change, wall volume increased most rapidly, in relation to cell volume, at the division site. The average volume of wall distal to the septum also increased but the slopes of the lines relating distal wall volume to cell volume were the same at all stages of the shape change. The quantity of wall per distal pole gradually declined with increase of cell volume and as the cells became more coccus-like. Collectively, these features suggest that wall continues to be produced at sites of cylindrical extension but fails to become incorporated into the existing cylinder to maintain a constant diameter. Instead, wall material may be used to thicken ...Continue Reading


Jan 1, 1987·Critical Reviews in Microbiology·R J Doyle, A L Koch

Related Concepts

Natto Bacteria
Cell Wall
Electron Microscopy

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.