PMID: 6981180Jan 1, 1981Paper

Quantitative studies of the interaction of 3H-dsDNA/anti-DNA immune complexes with complement: comparison and evaluation of the Raji cell, the solution phase C1Q, and the red blood cell linked complement fixation radioimmunoassays

Rheumatology International
R P TaylorT Conlon

Abstract

We have prepared antibody/3H-dsDNA immune complexes and have used three independent radioimmunoassays to quantitate their interaction with complement; the solution phase C1q assay, the Raji cell assay, and a complement-based red blood cell adherence assay (RBC-CF). Our results indicate that although there is reasonable qualitative agreement between the Raji cell assay and the RBC-CF assay, there are some differences in the quantitative range of sensitivities of the two assays. On the other hand, we find that most of the complement-fixing antibody/3H-dsDNA complexes are not detected in the solution phase C1q assay. The results suggest this is because the absolute concentrations of the immune complexes were too low to achieve significant precipitation under the standard conditions used in the C1q assay. The implication of these findings with respect to the potential detection and analysis of antibody/dsDNA immune complexes is discussed.

References

Jan 1, 1979·Advances in Immunology·A N Theofilopoulos, F J Dixon
Jan 1, 1976·The Journal of Clinical Investigation·A N TheofilopoulosF J Dixon
Aug 1, 1978·Clinical Immunology and Immunopathology·R D Sontheimer, J N Gilliam
Feb 1, 1973·Kidney International·V AgnelloH G Kunkel
Jan 1, 1974·Annual Review of Medicine·D Koffler
Mar 1, 1974·The Journal of Experimental Medicine·A N TheofilopoulosF J Dixon
May 1, 1981·Arthritis and Rheumatism·S J WallerM Johns
Jan 1, 1980·Journal of Immunological Methods·S E PedersenE L Wright

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibodies: Complement Activation

The complement system can be activated by antigen-associated antibody. In the classical pathway of complement activation, C1q, C4b, and C3b are all able to bind to the Fc portion of IgG or IgM. Find the latest research on antibodies and complement activation here.