Quantitative studies of the regular distribution pattern for Salmonella enteritidis in the internal organs of mice after oral challenge by a specific real-time polymerase chain reaction.

World Journal of Gastroenterology : WJG
Shu-Xuan DengZhen-Hua Zhang

Abstract

To identify and understand the regular distribution pattern for Salmonella enteritidis (S. enteritidis) in the internal organs of mice after an oral challenge over a 3 wk period. Assays based on the serovar-specific DNA sequence of S. enteritidis from GenBank, and a serovar-specific real-time, fluorescence-based quantitative polymerase chain reaction (FQ-PCR) were developed for the detection of S. enteritidis. We used this assay to detect genomic DNA of S. enteritidis in the blood and the internal organs, including heart, liver, spleen, kidney, pancreas, and gallbladder, from mice after oral challenge at different time points respectively. The results showed that the spleen was positive at 12 h post inoculation (PI), and the blood was at 14 h PI. The organism was detected in the liver and heart at 16 h PI, the pancreas was positive at 20 h PI, and the final organs to show positive results were the kidney and gallbladder at 22 h PI. The copy number of S. enteritidis DNA in each tissue reached a peak at 24-36 h PI, with the liver and spleen containing high concentrations of S. enteritidis, whereas the blood, heart, kidney, pancreas, and gallbladder had low concentrations. S. enteritidis populations began to decrease and were not ...Continue Reading

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Citations

Feb 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Robert W CrawfordJohn S Gunn
Apr 11, 2014·PloS One·Kathleen E DelGiornoAdrianus W M van der Velden
Aug 22, 2014·Journal of the Royal Society, Interface·Ayawoa S Dagbovie, Jonathan A Sherratt
Jun 12, 2018·Proceedings. Mathematical, Physical, and Engineering Sciences·A CangianiO J Sutton
Aug 28, 2012·Journal of Diabetes Science and Technology·Jeanne D JohnstonRickie Lee Marker-Hoffman

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